Histology week 6 (ch. 8&9, blood & lymphatic tissue)

What stain is this? What are its components?

Wright’s Stain

Methylene blue, eosin, and Azure

Blood components

What are A & B?

A: 55% plasma = water and plasma proteins

B: 45% formed elements = RBCs, WBCs, and platelets

Cellular blood components

What are A & B?

A: Buffy coat 1% = WBCs and platelets

B: RBCs = 99%

What is 7.2 um/

red blood cellls

What is this?

mature RBCs a nuclear biconcave disks

Capillary

What are A and B?

A: red blood cells

B: platelet

What is this?

Spherocytosis

What condition is this?

Sickle cell anemia

What stage of erythropoiesis is this?

Proerythroblast (1)

What stage of erythropoiesis is this?

Basophilic erythroblast (2)

What stage of erythropoiesis is this?

Polychromatophilic erythroblast (3)

What stage of erythropoiesis is this?

Orthochromatophilic erythroblast (4)

What stage of erythropoiesis is this?

Polychromatophilic RBCs reticulocytes (5)

What stage of erythropoiesis is this?

Red blood cells (6)

Which cell type is this? What stain is this?

Reticulocytes, new methylene blue stain

Reticulocytes

What are A, B, and C?

A: mitochondria

B: degenerating mitochondria

C: Golgi apparatus remnants

Which granulocyte is this?

Neutrophil

Which granulocyte is this?

Eosinophil

Which granulocyte is this?

Basophil

Which agranulocyte is this?

Monocyte

Which agranulocyte is this?

Monocyte

Which agranulocyte is this?

Lymphocyte

Neutrophil

What is D?

Drumstick Barr body

Neutrophil

What are A, B, C, D, and E?

A: specific granule

B: azure granule (Lysosome)

C: bacteria

D: antibody

E: phagolysosome

Neutrophil

What are A and S?

A: azure granules

S: specific granules

Which cell type is this?

Basophil

Basophil

What are A, B, C, D, E, F, and G?

A: Specific granule

B: receptor

C: antigen

D: antibody

E: degranlation

F: histamine

G: heparin

Basophil

What are S?

Large electron dense specific granules

Which cell type is this?

Eosinophil

Eosinophil

What are A, B, C, D, and E?

A: specific granule B

B: parasitic worm

C: antibody

D: release of specific granules

E: Ag-Ab complex degraded in phaholysosome

Eosinophil

What are A and B?

A: Bilobed nucleus

B: specific granules

What cell type is this?

Horseshoe monocyte

What cell type is this?

Kidney bean shaped monocyte

What cell type is this?

Small Lymphocytes

What cell type is this?

Large lymphocytes

What is this?

What are A and B?

Bone marrow mgakaryocytes

A: bone spicules

B: incipient platelets

What are the pink/arrowed cells?

Platelets

Lymphatic system

What are A, B, C, and D?

A: lymphocytes

B: extracellular (interstitial) fluid

C: foreign pathogen

D: lymphatic capillary

Lymphoid organs

What are A, B, C, D, E, F, and G?

A: Thymus

B: bone marrow

C: lymph nodes

D: spleen

E: Peyer’s patch \

F: appendix

G: tonsils

Lymphatic drainage

What are A and B?

A: drained by lymphatic duct

B: drained by thoracic duct

Lymph node architecture

What are A and B?

What are the yellow and purple?

A: Afferent lymphatic vessels

B: efferent lymphatic vessels

Yellow:: cortex

Blue: medulla

Cortex architecture

What are A, B, C, and D?

A: capsule

B: trabeculae

C: lymphoid nodule]

D: germinal centers

Cortex architecture

What are A and B?

A; cortex

B: deep cortex

Medulla architecture

What are A and B?

Medullary cords

Lymph node drainage

What are A, B, C, D, E?

A: afferent lymphatic capillaries

B: subscapular sinus

C: trabecular sinus

D: medullary sinus

E: efferent lymphatic vessel

What is this?

Reticular fibers in subscapular sinus

What is this?

Lymph node

Lymph node

What are A, B, C, D, and E?

A: capsule

B: outer cortex

C: deep cortex

D: trabeculae

E: germinal centers (secondary nodules)

What is this?

Germinal center, reaction center

Germinal center

What are A, B, C, D?

What are the red circles?

A: small lymphocytes (mantle)

B: follicular dendritic cell

C: lymphoblasts

D: small sized lymphocytes

Red: mitosic figures

Lymph node

What are A, B, And C?

A: outer cortex

B: deep cortex:

C: medulla

What is this?

High endothelial venue (HEV)

Medulla

What are A, B, C, D, E, and F?

A: artery

B: medullary sinus

C: Medullary cord

D: Capillary bed

E: HEV

F: vein

Lymphatic vessels

What are A and B?

A; eosinophilia lymph

B: valves

What tissue is this? What stain is this?

Lymph node medulla, silver stain

What tissue is this? What stain is this?

Lymph node medulla, India ink + H&E

Spleen

What are A, B, C, D, E, F, and G?

A: capsule

B: trabeculae

C: red pulp

D: white pulp

E: splenic nodule (white pulp)

F: germinal centers

G: central artery

Trabeculae

What are A, B, C, D and E?

A: trabecular artery and vein

B: peripheral white pulp (b lymphocytes)

C: germinal center

D: central artery

E: White pulp PALS (T lymphocytes)

Trabeculae

What are A, B, and C?

A: trabecular vein

B: trabecular artery

C: trabeculae (dense irregular connective tissue)

Periarterial lymphatic sheath (PALS)

Longitudinal section

What are A and B?

A: PALS (T cells)

B: central artery

Splenic nodule with germinal centers G

Cross section

What are A, B, and C?

A: PALS (T cells)

B: Central artery

C: Germinal center (B cells)

Closed splenic circulation

What are A, B, C, D, E, F, G, and H?

A: Trabecular artery

B: PALS

C: germinal center D

D: pennicillar arteries

E: splenic sinousoid (capillary)

F: pennicillar venule

G: Central artery

H: central vein

Open splenic circulation

What are A< B, C, D, E, F, G, H, and I?

A: trabecular artery

B: PALS

C: Germinal center

D: splenic sinousoids (capillaries)

E: pennicillar arterioles

F: macrophages

G: Penicillar venules

H: central artery

I: central vein

Red pulp H&E

What are A, B, and C?

A: macrophages

B: splenic sinus

C: splenic cord

Thymic structure

What are A, B, C, D, and E?

A: capsule

B: Septae

C: Cortex

D: medulla

E: lobules

Thymic structure

What are A and B?

What are the arrows?

A: macrophages

B: epithelial reticular cells

Arrows: Hassal’s corpuscles