Other Related Information April - August

What happens if you strip and reprobe a membrane too much?

You wash away your target protein and ruin your data accuracy

How does sonication improve cell lysate prep?

Using high-frequency sound waves to mechanically break open stubborn cell membranes and shear long, viscous DNA strands, decreases viscosity

Is TTT Heterotrimeric?

Yes

What makes TTT heterotrimeric?

Heterotrimeric proteins are marcomolecules composed of three distinct polypeptide subunits, at least one has to differ from the other two

What’s the difference between a homotrimer and a heterotrimer?

Homotrimers are composed of three identical subunits

What are all the proteins and kinases TTT is a chaperone for?

1. ATM

2. ATR

3. DNA PKcs

4. mTOR

5. SMG1

6. TRRAP

7. PIKKs

8. other proteins anywhere from 300 - 500 kDa in size

What’s the kinome?

All the protein kinases in a genome

How many protein kinases do humans have?

518

What was the first subunit discovered of TTT?

TEL2

What organism was TEL2 discovered in?

In c.elegans 40 years ago

What happens when TEL2 is deleted?

TEL2 deletion reduces the protein levels of all PIKKs

TEL2 acro. stands for

Telomere maintenance 2

TTI1 acro.

TEL Two Interacting Protein 1

What cellular checkpoints are TTI1 and TTI2 required for?

The establishment of S and G2/M checkpoints

DNA-PKCs

DNA dependent protein kinase catalytic subunit, repair DSB by NHEJ

SMG1

Suppressor with morphological effect on genitalia 1, regulates nonsense mediated decay of aberrant mRNA

mTOR

Mammalian target of rapamycin, controls cell growth in response to environmental cues

TRRAP

The catayticaly inactive transformation/transcription domain associated protein, regulates gene expression via histone acetyltransferase complexes

What does it mean for one gene to be allelic with another?

It means there are different variations or versions of the same gene

Pleiotropy

A single gene or mutation influences multiple unrelated phenotypic traits or body systems

What kind of effect to TEL2 mutants have in different organisms?

A pleiotrophic effect