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Where does transcription occur in prokaryotes and eukaryotes?
Prokaryotes: cytoplasm; eukaryotic nuclear genes: nucleus.
How does transcription initiation differ between bacteria and eukaryotes?
Bacteria generally use sigma factor; eukaryotic protein-coding genes use multiple general transcription factors.
How many principal RNA polymerases do bacteria and eukaryotes have?
Bacteria use one principal type; eukaryotes have RNA polymerases I, II, and III among others.
What does RNA polymerase I produce?
Most major rRNAs, excluding 5S rRNA.
What does RNA polymerase II produce?
Pre-mRNAs from nuclear protein-coding genes.
What does RNA polymerase III produce?
tRNAs, 5S rRNA, and some other small RNAs.
How do bacterial operons differ from typical eukaryotic transcription units?
Bacterial operons can transcribe several related genes together; eukaryotic protein-coding genes are generally transcribed separately.
Why can transcription and translation occur simultaneously in bacteria?
Bacteria lack a nucleus, so ribosomes can translate mRNA while it is being transcribed.