Unit 7: Supportive, Selective, and Differential Media

What media type is Chocolate?

Supportive

What media type is MacConkey?

Selective

What media type is TSA-Blood

Differential

What media type is CNA-Blood?

Selective and Differential

What does supportive media mean?

grows wide variety of fastidious bacteria

Does Chocolate have a substrate, enzyme, indicator or predicted product?

no

What is MacConkey Selective for?

crystal violet bile salts gram -

What is MacConkey Differential for?

Lactose

What is TSA differential for?

different types of hemolysis

What is CNA selective for?

colistin nalidixic acid and inhibit growth of gram -

What is CNA differential for?

different types of hemolysis

What is Chocolate supportive for?

NAD hemin fastidious microbes

What is the enzyme for MacConkey?

lactase

What is the substrate for MacConkey?

lactose

What is MacConkey product?

Acids

What is MacConkey indicator?

neutral red

What is MacConkey enzyme positive color?

fuchsia pink

What is TSA substrate?

whole RBC

What is TSA enzyme?

hemolysins

What is TSA product?

lysed RBC

What is CNA substrate?

whole RBC

What is CNA enzyme?

hemolysins

What is CNA product?

lysed RBC

What is Alpha hemolysis?

partial greenish agar

What is Beta hemolysis?

complete clear agar

What is Gamma hemolysis?

no change agar

If no growth of bacteria or colonies show on TSA or CNA can tell if bacteria produces lactase?

no need growth