AP Bio Glycolysis

Products and Enzymes

Purpose of Glycolysis

Break down Glucose into 2 Pyruvate molecules

Where does glycolysis happen?

cytosol of cell

What steps are considered the Energy Investment Phase and why?

Steps 1-5

ATP is being used

What steps are considered the energy payoff phase and why?

steps 6-10

ATP is being made

Glucose → ____ with enzyme ____ ;

uses 1 ___ producing 1 ___

Glucose 6-phosphate (G6P)

Hexokinase

1 ATP → 1 ADP

Glucose 6-phospate (G6P)→ ____ with enzyme ____

Fructose 6-phosphate (F6P)

Phosphoglucoisomerase

Fructose 6-Phosphate → ____ with enzyme ____;

uses 1 ___ producing 1 ___

Fructose 1,6-bisphosphate (FBP)

Phosphofructokinase

1 ATP → 1 ADP

Fructose 1,6-bisphosphate → ____ and ____ with enzyme ____

Glyceraldeyhde 3-phosphate (G3P) and Dihydroxyacteone phosphate (DHAP)

Aldolase

Interconversion of ___ and ___ using enzyme ___

G3P and DHAP

Triosephosphate isomerase

Glyceraldehyde 3-phosphate (G3P) → ____ with enzyme ____ ;

uses 2 ___ producing 2 ___

adds 2 __

1,3-bisphoglycerate

Glyceraldeyde-3-phosphate Dehydrogenase (GAPDH)

2 NAD+ → 2 NADH

P groups

1,3-bisphoglycerate → ____ with enzyme ____ ;

uses 2 ___ producing 2 ___

3-phosphoglycerate

Phosphoglyceratekinase

2 ADP → 2 ATP

3-phosphoglycerate → ____ with enzyme ____

2-phosphoglycerate

phosphoglyceromutase

2-phosphoglycerate → ____ with enzyme ____ ;

loses 2 ___

Phosphoenol-pyruvate (PEP)

Enolase

2 H2O

Phosphoenol-pyruvate (PEP) → ____ with enzyme ____ ;

uses 2 ___ producing 2 ___

Pyruvate

Pyruvate Kinase

2 ADP → 2 ATP

Net Total of Glycolysis per 1 glucose molecule:

__ ATP

__ Pyruvate

__ NADH

__ H₂O

Net Total of Glycolysis per 1 glucose molecule:

2 __

2 __

2 __

2 __