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what color tube to use
blue (sodium citrate)
required ratio
1 part citrate to 9 parts whole blood
processing and storage
centrifuge at 2500 for 15 mins to get citrated-platelet poor plasma (PPP)
Test within 2-4 hrs or freeze plasma immediately
sample collection
as little trauma as possible, prefer clean stick
tube should be plastic (glass can trigger clotting cascase)