Glycolysis Enzyme Purpose

Net equation of glycolysis

1 Glucose +2 P +2 ADP +2 NAD+ → 2 Pyruvate + 2ATP + 2NADH + 2H+ +2H2O

Hexokinase

adds first phosphate and traps glucose in cell

Phosphoglucose Isomerase

converts an aldose to a ketose so three carbon products will be formed in the aldol cleavage reaction

Phosphofructokinase (PFK)

adds a second phosphate so that each product of aldol cleavage will have one

Aldolase

cleaves a 6 carbon molecule (F-1,6-BP) into 2 rapidly interconvertible isomers (DHAP and GAP)

Triose Phosphate Isomerase

rapidly interconverts two, three carbon isomers (DHAP to GAP) to provide a steady supply of GAP to stage 2

Glyceraldehyde 3-Phosphate Dehydrogenase

produces a high phosphoryl transfer compound

Phosphoglycerate Kinase

produce ATP through substrate level phosphorylation

Phosphoglycerate Mutase

works towards making a high phosphoryl transfer potential compound (moves phosphate from C2 to C3)

Enolase

create high phosphoryl transfer potential compound

Pyruvate Kinase

produce ATP through substrate level phosporylation