Hemoglobin Hematocit and Buffy Coats

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Last updated 9:54 PM on 5/21/26
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38 Terms

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What is the function of hemoglobin?
Transport oxygen to tissues and carbon dioxide to lungs
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Why is hemoglobin preferred over RBC count?
Better indicator of anemia
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What is the structure of hemoglobin?
Four alpha chains, four beta chains, and four heme groups
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What is oxyhemoglobin?
Hemoglobin bound to oxygen
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What is carboxyhemoglobin?
Hemoglobin bound to carbon monoxide
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What is methemoglobin?
Hemoglobin with iron in Fe3+ form
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Why can't methemoglobin bind oxygen?
Iron is oxidized to Fe3+
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What is sulfhemoglobin?
Hemoglobin irreversibly bound to sulfur
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What is cyanmethemoglobin?
Hemoglobin combined with cyanide for measurement
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What method became the reference for hemoglobin?
Cyanmethemoglobin method
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What is the principle of cyanmethemoglobin method?
Oxidation of hemoglobin and conversion to HiCN
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What reagent oxidizes hemoglobin?
Potassium hexacyanoferrate
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What happens to Fe2+ in hemoglobin?
Converted to Fe3+
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What forms after cyanide is added?
Cyanmethemoglobin (HiCN)
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What hemoglobin type converts slowly?
Carboxyhemoglobin
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How can COHb affect results?
Cause falsely low hemoglobin
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What hemoglobin type is not measured accurately?
Sulfhemoglobin
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Why is sulfhemoglobin not included?
Different absorbance properties
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What specimen is used for hemoglobin testing?
EDTA whole blood
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What causes falsely increased hemoglobin readings?
Turbidity
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What conditions cause turbidity?
Lipemia, high WBC, high platelets
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What effect do lyse-resistant RBCs have?
Increase turbidity
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What is hematocrit?
Volume of RBCs in whole blood
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What is another name for hematocrit?
Packed cell volume (PCV)
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What is the buffy coat?
Layer of WBCs and platelets
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Where is buffy coat located?
Between RBCs and plasma
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What happens to hematocrit after blood loss?
Temporarily low
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Why is hematocrit unreliable after blood loss?
Plasma replaced faster than RBCs
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What effect does dehydration have?
Falsely increases hematocrit
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What RBC abnormalities increase hematocrit?
Sickle cells, macrocytes, spherocytes
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Why do abnormal RBCs increase hematocrit?
Trap plasma
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How does excess anticoagulant affect hematocrit?
Decreases due to shrinkage
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What does IV contamination cause?
Falsely decreased hematocrit
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What is the purpose of buffy coat preparation?
Concentrate WBCs and abnormal cells
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When is buffy coat used?
Low WBC counts
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What tubes are used for buffy coat?
Wintrobe or capillary tubes
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What is buffy coat used to detect?
Abnormal cells or microorganisms
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Why is buffy coat useful for infections?
Concentrates infected cells