MT 124 SEM Hemorrhagic and Thrombotic Disorders

Seminar Laboratory Diagnosis on Hemorrhagic and Thrombotic Disorders

12, 11, 9, 8,10, 5 2, PK, HMWK

APTT clotting factors detected

12, 11, 9, 8,10, 5 2, PK, HMWK

Clotting Time Clotting factors detected

8

vWF Assay Clotting factors detected

12, 5, 7, 10

PT Clotting factors detected

10

Strypven Time

1

TCT/TT (Thrombin Clotting Time/ Thrombin Time clotting factors detected

1

HDTT (High Dose Thrombin Time) clotting factor detected

1

Reptilase Time clotting factor detected

8 or LA

Bethesda Assay clotting factor detected

10 or LA

Dilute Russel’s Viper Venom test clotting factor detected

5-10 UG/ML (agglutination)

Latex FDP Assay RV

500 NG or 0.5 UG/ML (Agglutination)

D-dimer Assay RV

No Paracoagulation or Polymerization

Protamine Sulfate Dilution Test RV

No Agglutination

Tanned Red Cell Agglutination Test RV

0.75 - 9 UG/ ML (Agglutination)

Staphylococcal Clumping Test RV

Bleeding Time

time it take the blood to stop the flow after a puncture/injury

< 9 minutes

duke’s and copley RV

7-15 minutes

ivy RV

Brecker and Cronkite

gold standard for plt count

Aggregometry

test where platelet agonist/ compounds which enhance platelet aggregation will be added to the sample

Positive Responses in all agonist

Aggregometry normal/reference result

85% (factor 8)

Hemophila A

14% (Factor 9 -hindus)

Hemophila B / Christmas Disease

Hemophila C / Rosenthal syndrome

1% (Factor 11 - jews)

Prolonged Reptilase Time

confirmatory test for liver hemorrhagic disease

Atresia

Biliary tract obstruction

9, 10, 7, 2

Dysfunctional Factors in Vit. K deficiency

8, 2, 5, 9, 13 & vWF

Auto anti-VIII Inhibitor inhibits what factor/s?