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objects microscopic when…
they need to be magnified for the human eye to be able to see them.
magnification -
refers to process of making objects appear larger than they are
resolution -
ability to see objects clearly enough to tell them apart
limit of resolution in human eye is…
.1mm or 100 um
ocular lenses magnification is…
10X
4X objective lenses magnification is…
40X
10X objective lenses magnification is…
100X
40X objective lenses magnification is…
400X
100X objective lenses magnification is…
1000X
unicellular -
composed of one cell
multicellular -
composed of many cells
eukaryotic cells -
have nucleus and organelles
prokaryotic cells -
DO NOT have nucleus or organelles
working distance -
distance between lens and slide
field of view -
circular area seen when looking though scope
depth of field -
thickness of specimen that is in sharp focus
parcentric -
center of the field of view
parfocal -
when scope is in focus with one lens in place, the same stage position will be in focus with other lenses
bacillus morphology is…
rod shaped
coccus morphology is…
round/spherical shaped
spirillum morphology is…
corkscrew shaped
single arrangement is…
1 cell
diplo arrangement is…
2 cells
Palisades arrangement…
looks like a “picket fence”
tetrad arrangement is…
4 cells
sarcina arrangement is…
8 cells
strepto arrangements are…
cells in a chain
staphlo arrangements are…
clusters
ocular piece -
eyepiece
objective lenses -
lenses close to stage (4X, 10X, 40X, and 100X)
coarse adjustment knob -
larger, outermost knob that moves stage toward and away from objective lenses
fine adjustment knob -
smaller knob within course knob used for finer focusing
stage -
platform where slide is placed
illuminator -
light source at bottom of scope below the stage
condenser/iris diaphragm assembly -
directly beneath stage
base -
bottommost part of scope that contains illuminator
slide holder -
metal clip on stage the secures slide
arm -
positions objective lenses and ocular above the stage