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What does EPT stand for?
Ephemeroptera (mayflies), Plecoptera (stoneflies), and Trichoptera (caddisflies), three orders of benthic insects that are highly intolerant to pollution
A ___ is the fundamental unit of taxonomy
Species
How do older systems of taxonomic classification compare to newer ones?
Older systems classify based on morphological characteristics, while speciation is now done based on genetic comparison
How much variation in DNA base pair sequences is needed to grant separate species status? How much for RNA
30% in DNA, 5% in RNA
Which genes are generally used for identifying both prokaryotic and eukaryotic organisms?
16S rRNA and 18S RNA
How is RNA amplified for microbial diversity measurements?
Amplification using PCR
Fingerprinting techniques (Ex. DGGE, T-RFLP)
Define: Lateral gene transfer
The transfer of genes between two organisms by means other than reproduction
Why is ribosomal RNA the best candidate for speciation sequencing?
Because they do not laterally transfer genes
What are the three methods of lateral gene transfer?
Transduction: Bacteria takes up loose DNA from the environment
Transformation: A virus transfers DNA from one bacterium to another
Conjugation: Two bacteria transfer DNA through contact
Bacteria and Archaea are two groups with maximum ____ diversity, while Eukarya has maximum ____ diversity
Metabolic, morphometric and behavioral
Define: Commensalism
An interaction between two species in which one benefits, and the other is neither harmed nor helped
Define: Ammensalism
An interaction between two species in which one is harmed, and the other is neither harmed nor helped
Most aquatic vertebrates are ____
Bony fishes
How big are viruses?
250 - 350 nm
Viruses have been demonstrated to travel up to ___ deep into groundwater
50m
What are some characteristics of Archaea?
Single-celled, non-nuclear, and non-organelle organisms
Significantly different from bacteria in cell wall structure, and in metabolic processes
Live in all environments, including hot springs, saline lakes, and anaerobic waters
What is the most diversified organism?
Bacteria
Why are the two types of cells produced by cyanobacteria/blue-green algae
akinetes (resting spores) and heterocysts (site for nitrogen fixation)
Less than ___ of all bacteria have been identified
1%
What are some characteristics of bacteria
Single-celled, non-nuclear, and non-organelle organisms
What are some characteristics of protists?
Single celled, heterotrophic organisms (except for protophyta which has chlorophyll)
Significant members of the nutrient cycle, material degradation, energy transfer, and causing disease
What is Chrysophyceae?
Golden algae (Ex. Dynobryon), considered facultative heterotrophs (shifting between photosynthesis and ingestion)
What is Dinophyceae?
Dinoflagellates , unicellular, freeswimming, has cellulose plates and two flagella, can ingest other organisms or do photosynthesis. Can be toxic
What is Bacillariophycea
Have a silicate cell wall, called a frustule. Frustrules don't dissolve and get settled in sediments, acting as a proxy to analyze eutrophication history
Have elongate, pennate, and circular centric forms
What is Euglenophyceae?
Euglenids, a group of eukaryotic flagellates, can be pigmented or colourless, autotrophic or heterotrophic, exhibits amoeboid cell movements
What is Chlorophyceae?
A type of green algae, single or multicellular, generally found floating or attached to surfaces
What is Charophyceae?
Stoneworts, considered the evolutionary precursor of most land plants. Can become abundant in productive wetlands, indicative of nutrient pollution
What are some characteristics of Protozoans?
Can be auto or heterotrophs, small to large
Can ingest organic particles, viruses, bacteria, and in some cases diatoms
Some make cysts, and are highly resistant to environmental extremes
Some are parasites to humans, and other organisms such as fish
Can be flagellate, ciliate, and amoeboid depending on their mobility
What group of fungi are mostly aquatic?
Oomycota
There are more than ___ species of aquatic fungi
600
Where are aquatic lichens found?
In wetlands, especially boreal and temperate, but never in groundwater
What are the three growth patterns/forms of aquatic lichens?
Foliose (leaflike)
Fruticose (finger-like projections)
Crustose (appressed to solid surfaces)
Plants in water are called ____
Macrophytes
Traditional categories of aquatic vascular plants include
Floating unattached
Floating attached
Submersed
Emergent
What are some characteristics of the Phylum Porifera
Sponges, filter feeders
Exhibit sexual and asexual (via fragmentation, or production of gemmules) reproduction
Most are marine, but 35 species are found in freshwater
What are spongillaflies?
Insects that prey on sponges, by piercing the sponge body to feed on its internal fluids
What are some characteristics of the Phylim Cnidaria
Includes jellyfish, anemones, corals, and hydroids
Mostly marine (hydrozoans are freshwater)
Exhibit alternation of generations
What is the most primitive organism?
Sponges
What are the three classes of the Phylum Platyhelminthes?
Turbellaria (free living worms)
Trematoda (flukes)
Cestoda (tapeworms)
Schistosomiasis is caused by __
Shistoma, a type of fluke which needs both humans and snails to complete its life cycle
What are some characteristics of the Phylum Gastrotricha?
Also called hairy-back worms, microscopic & ciliated
Abundant in freshwater, but not well studied
Eggs can survive extreme conditions (freezing, heat, anoxic environments, drying, etc) and be viable for years
What organisms belong to the Phylum Nemertea?
Ribbon worms
What are some characteristics of the Phylum Rotifera?
2000 species in freshwater and more in marine
Produces eggs capable of withstanding harsh conditions
Includes Bdelloids, the largest metazoan without sexual reproduction
What are some characteristics of the Phylum Nematoda?
Non-segmented worms living in benthic habitats
Feeds on detritus, algae, and parts of organic plants
Includes 2000 reported freshwater species
What organisms belong to the Phylum Nematomorpha?
Horsehair/Gordian worms
What are some characteristics of the Phylum Mollusca?
One of the most abundant freshwater invertebrates
Includes several invasive species (Ex. zebra and quagga mussels)
Bodies have both hard and soft components
What are some characteristics of unionoid mussels?
The most diverse freshwater bivalves found in North America
Used to make buttons
Long lifespan: 6-100 years
Where are zebra mussels native to?
The Caspian Sea
How was the zebra mussel introduced into North America?
Via ballast water in the 1980s
What are some characteristics of the Phylum Anellida?
Includes segmented worms, with tubular bodies and complete digestive tract
Sexual and asexual reproduction, includes some hermaphrodites (Ex. earthworms)
What organisms belong to the Phylum Bryozoa?
Simple, sessile invertebrates living in colonies
Can be encrusted or stalked
What are water bears?
Microscopic, bilaterally symmetrical organisms, that reproduce via parthenogenesis
What are the three main phyla within the group Arthropoda?
Chelicerata (water mites and spiders)
Crustacea (Crayfish, shrimp, amphipods, and isopods
Unirama (insects and collembola)
What is the most diverse & successful group of organisms?
Arthropoda
What is the most primitive form of fish?
Lampreys
What is the difference between anadromous and catadromous?
Anadromous (born in freshwater): Adults move from saltwater to freshwater to spawn (Ex. Salmon)
Catadromous (born in saltwater): Adults move from freshwater to seawater to spawn (Ex. Eels)
What are some examples of tetrapods?
Amphibians, reptiles, birds, and mammals
Define: Species diversity
A measure of the number of species present in an area (richness) and how evenly members are distributed within the habitat (evenness)
What is the most common diversity index?
The Shannon Index
North America’s freshwater habitats are divided into ____ ecoregions
76
The ultimate source of biological diversity is ____
Evolution
____ is the most studied ancient lake
Lake Baikal in Russia
What are Hypogean fauna? Why do they have so many unique endemic organisms?
Interior fauna of soils, which have many endemic organisms because there is such a low chance of disturbance
What kinds of habitats usually favour high numbers of endemic species?
Ancient lakes, isolated ponds, river basins with continuous existence, groundwater aquifers with large channels, hypogean environments
What factors influence the distribution of species?
Species interactions and introductions, productivity, habitat type, dispersal ability, and colonization
What is the formula of the species - area relationship
S = cA^z
Where S - # of species, A - area, c - constant (number of species / unit area), and z = slope
Define: Succession
Sequential changes of species dynamics until reaching a climax community
Define: Minimum viable population
The lowest number of individuals required for a population to survive in the wild
What phyla is faces the largest threat of extinction in recent times?
Amphibians
What is the formula for the Shannon diversity index?

Define: Continental water systems
Water bodies contained within continents, as opposed to oceanic systems which separate continents
What are some ways in which continental water systems can be formed
Topographic depressions, runoff and run-in, rain, volcanic eruptions leaving craters, etc.
Define: Limnology
The study of continental waters
Why do volcanic crater lakes have acidic conditions?
Due to the deposition of volcanic gases
What did Dr. Evelyn Hutchison do for modern day limnology?
Wrote A Tretise on Limnology, a comprehensive guide to studying inland waters
What are the three major compartments of water in the hydrologic cycle?
Oceanic, atmospheric, and land systems
Define: Fluxes
The movement of water along different compartments
The global renewable supply of water is about , ___ humans use __ of accessible runoff
39 000 km^3 / year, 54%
What is the largest freshwater aquifer in NA?
The Ogallala aquifer
What sector uses the most water in Canada?
Industrial (91.7%), specifically electric power (70% of that)
How much water does Canada use per year (in cubic M)
36.1 billion cubic m
What are the two groups of properties that make water what it is?
Physical: Dictates how water acts as a solvent, and how density varies
Chemical: Composition of the water molecule allows it to stay water in a wide range of temperatures.
What kinds of bonds are present in water molecules?
Covalent bonds, hydrogen bonds
The maximum density of water occurs at __
4 degrees c
With increasing depth and pressure of water, ___ increases
Solubility
What are some of the properties of water?
High heat capacity, boiling and melting points, high surface tension, high heat of vapourisation, excellent solvent for ions and polar molecules
Define: Viscosity
Fluidity, resistance to change from one form to another
Define: Inertia
Resistance to a change in state of motion
What is the purpose of Reynold’s number?
Helps quantify spatial and velocity-related effects on viscosity and inertia, helps understand the effects of water and its movement on living organisms
What is the formula for Reynold’s number?
Re = Fi / Fv (Fi = inertial force, Fv = viscous force)
Chemical diffusion is affected by:
Concentration gradients, advective transport, temperature, nature and size of molecules, interference by living organisms, etc.
____ surface area leads to more diffusion
Higher
Diffusion of materials in water is described by ___ law
Fick’s law: Diffusion flux is +vely related to the diffusion coefficient, the concentration gradient, and the reciprocal distance between the source and location at which diffusion occurs
Light transparency of water bodies can be measured by a ____
Secchi disk
Define: Hydrodynamics
Temporal and spatial movement and distribution of water in the environment
Define: Physiography
Physical geography
Define: Ecohydrology
Hydrology in relation to ecology
What are the two types of scale to consider when studying an organism?
Temporal and distance (Ex. how often does the organism reproduce, what is its lifespan, how big is it, how big is its habitat)
What is the difference between infiltration and percolation?
Infiltration occurs near the surface, percolation occurs underground
When the quantity of water exceeds the ____ (beyond soil saturation) water runs off
matric potential