Blood Bank Medialab

The lectin Dolichus biflorus agglutinates which types of cells

A1B

Transfusion-related acute lung injury (TRALI) is a serious blood transfusion complication that can be characterized by all of the following except

increased central venous pressure

What would be considered a common characteristic of anti-Jka and anti-Jkb

the antibodies show dosage with Kidd antigens on red cells

ABO antibodies are classified as which of the following two immunoglobulin classes

IgG and IgM

Which of the following is true regarding the Lutheran blood group system

Lub is a high frequency antigen

A Bombay individual's blood specimen can be differentiated from a blood specimen of a group O person by which of the following

testing with anti-H lectin (Ulex eruopaeus)

If Jka is showing dosage, how might reactions on an antibody panel appear

Weaker if heterozygous for Jka and Jkb

Why is it dangerous to transfuse a blood group O person with a unit of blood group A

The patient's anti-A would destroy the donor's cells with severe consequences to the patient

A woman with the blood type AB will show antigens for both the A and B blood groups. This is an example of what type of inheritance

codominance

A primary immune response typically presents with which class of antibody

IgM

Which of the following blood components contains the most factor VIII concentration relative to the unit volume

cyoprecipitated AHF

What is the minimum volume (milliliters) of Rh positive red blood cells that would be needed to produce anti-D in an Rh negative individual

< 0.1 mL

If an individual has blood type O, which of the following are possible genotypes

OO only

When a unit of packed RBC's split using the open system, each portion of the unit must be issued

within 24 hours

An exchange transfusion has been ordered for a low birth weight infant. The physician has ordered irradiated Red Blood Cells for this purpose. Why is it necessary to irradiate the blood products for this patient

to prevent transfusion-associated graft-versus-host disease (TA-GVHD)

When a unit of packed RBC's is split using the open system, each portion of the unit must be issued

within 24 hrs

Which of the following ABO blood groups has the highest number of H antigen sites on the RBC membrane

Group O cells

What is the minimum acceptable hematocrit level for a male that wants to donate double RBC units through RBC apheresis

40%

Donor and recipient blood samples must be kept for at least how long after transfusion

7 days

Which of the following patients are at risk for transfusion-associated graft versus host disease (TA-GVHD) and require irradiated cellular blood products

immunocompromised patients including neonates less than 4 months of age and patients receiving chemotherapy

What is considered the definition of allogeneic blood

donated by a donor for a recipient other than the donor to use

Compared to plasma frozen within 8 hours of collection, plasma frozen within 24 hours of collection will likely have reduced levels of Factor

VIII

The immediate spin phase of an indirect antiglobulin test (antibody screen and antibody identification) could most likely lead to the detection of which of the following

clinically insignificant cold antibodies

Leukocyte-reduced blood products may be administered to reduce the risk of febrile reactions and what disease or condition

cytomegalovirus

Which is in the correct order from the lowest concentration of H antigen to the highest concentration of H antigen

Bombay, A1B, A2, A2B, B, A1, O

Compared to the primary immune response, the secondary immune response typically demonstrates

more antibodies produced

Which Lewis antigen(s) would be detected when phenotyping the red cells of an adult who has the Le, Se, and H genes

Leb

What are the possible ABO genotypes of offspring from parents whose genotypes are OO and AB

OA and OB

Which blood group is considered the "universal" donor when transfusing Red Blood Cells

Group O

What is the minimum hemoglobin concentration required for autologous blood donation

11 g/dL

Which of the following noninfectious complications of blood transfusion is prevented by the irradiation of blood components

transfusion-associated graft versus host disease (TA-GVHD)

Donor red blood cells (RBCs) were collected on July 12th of this year. The RBCs where then frozen in glycerol on July 17th (5days later) and stored at -65C.

What should the expiration date read on the frozen RBC unit

July 12th: 10 years from collection date

Which of the following packed RBCs could be transfused to a group O patient

Group O

Which of the following group B antigens is generally associated with a mixed field reaction

B3

Persons who have received a dura mater transplant are not eligible to donate blood and are permanently deferred. This is due to an increased risk of which of the following

CJD or vCJD

what is the most common treatment for hemolytic disease of the fetus and newborn (HDFN) due to ABO incompatibility

phototherapy

What is the most common Rh haplotype among whites

DCe

Donation of which apheresis blood product more than once every four weeks requires monitoring of total plasma protein and antibody levels

plamapheresis

The addition of Low Ionic Strength Solution (LISS) to the testing environment when performing an indirect antiglobulin test is designed to do what

lowering the zeta potential

Which blood group is considered the universal recipient for transfusion of red blood cells

AB

Following a major trauma event, a family of our walks into your donor center hoping to donate blood. Which one of the following individuals may donate blood today

A 22-year-old woman using oral contraceptives

Antibodies directed against granulocyte antigens have been implicated for all of the following except

acute hemolytic transfusion reactions

Which of the following describes the main function of hemoglobin

carry oxygen

All of the following are major Rh antigens, except

d

In an emergency release, why do blood bankers seldom encounter patients who have experienced hemolytic transfusion reactions (HTR) of uncrossmatched packed RBCs

the incidence of unexpected red blood cell antibodies is relatively low

Following a motorcycle accident, a man sustains heavy bleeding and is given two units of blood by your blood bank. The patient does not demonstrate any antibodies prior to the transfusion and cross matching the unit did not demonstrate incompatibility. Unfortunately, seven days later he is still anemic and has now developed a fever and jaundice. Antibody screening of a new specimen from this patient seven days after the transfusion reveals the presence of an antibody

Anti-Jka

The presence of hemolysis in a post-transfusion blood sample is best associated with which of the following

ABO incompatibility

Antibodies are ruled out using panel cells that are homozygous for the corresponding antigen because

stronger reactions are seen with homozygous cells

Which one of these Lewis blood group system phenotypes usually produces anti-Lea

Le(a-b-)

The most significant blood group antibodies belong to which immunoglobulin classes

IgG and IgM

What is the rare phenotype found exclusively in male patients that is caused by X-linked inheritance from a carrier mother, often demonstrating a chronic but well-compensated anemia as well as muscle and nerve disorder

McLeod

What is the required storage temperature for thawed cryoprecipitate

20-24 C

What procedure utilizes leukapheresis to collect the buffy coat from the whole blood

photopheresis

What step must be performed before a rejuvenated blood unit may be transfused to the patient

the unit must be washed

A rejuvenation solution may be used up to how many days following expiration of a red cell unit

3 days

Which of the following best describes a transfusion reaction

any adverse event associated with the transfusion of blood components

Louisa is a healthy 24-year-old woman that wants to donate blood today. Her hemoglobin, temperature, blood pressure, and pulse are all within the acceptable limits for donating. She's in good health and not taking any medications. She recently divorced her spouse who was a hemophiliac that regularly received factor concentrates. Can Louisa donate blood today

Maybe, depending on Louisa's last sexual contact with her ex-spouse

Which type of antibody can cause HDFN in any pregnancy (first or subsequent), but is usually limited to less severe symptoms

Anti-A,B

Lewis Blood Group System is a human blood group unlike most others. The antigen is produced and secreted by exocrine glands, eventually adsorbing to the surface of red blood cells. Its expression is based on the genetic expression of the Lewis and Secretor genes.

Based on the following genotype (Le) (Se), what would you predict the Lewis antigen phenotypic expression to be

Le(a- b+)

Which of the following conditions is most frequently associated with anti-I

cold agglutinin disease

When is testing for weak D optional

testing for weak D on potential transfusion recipient samples

For which of the following antibodies is the DAT most likely to be negative when testing a newborn for possible HDFN

Anti-A,B

What blood system is often tested to either establish or dispute paternity

HLA

Which of the following is the most prevalent blood type found in the United States

O positive

Hemolytic anemias are diagnosed by clinical findings and laboratory test results. All of the following are lab values that are relied on to help diagnose hemolytic anemia except

fibrinogen and C-reactive protein

How long after potential donors have diagnosed with malaria should they be deferred from doing a blood donation

3 years

Which of the following is the most common type of autoimmune hemolytic anemia

warm autoimmune hemolytic anemia

To prevent Rh alloimmunization is pregnancy between mother and fetus, it is recommended to initiate RhIG prophylaxis when

Rh-negative pregnant females, antenatal is the third trimester

All of the following can be indications of anemia and the potential need for a tranfusion of packed red cells except

increased haptoglobin

What is the cause of most severe acute hemolytic transfusions reactions

ABO incompatibility

What should be done first if a donor unit of red blood cells is found to be incompatible at the antiglobulin phase but compatible at immediate spin with several different recipients

perform a direct antiglobulin test (DAT) on the donor unit

Enzyme panel reactions cannot be used as the only source of rule-outs during antibody identification. Why is this

some antigens are destroyed and may not detect an antibody that is present

Which of the following antibodies are enhanced using enzyme panels during antibody identification in the blood bank

Rh

A patient transfused with two units of packed cells spiked a fever of 99.5 F and complained of chills five days after transfusion. The direct antiglobulin test (DAT) was positive with anti-IgG, but negative with anti-C3b. Compatibility testing was performed on the pre- and post-transfusion specimens. The post-transfusion specimen was incompatible with one of the donor units transfused. An antibody screen was done on both the pre-and post-transfusion specimens. An antibody was detected in the post-transfusion specimen only and identified by panel studies as anti-Jka. This transfusion reaction is most likely

a delayed hemolytic transfusion reaction

Which occurrence is a medical error

incompatible blood is transfused into a patient during surgery

If an autologous blood component is shipped from the donor center without the completion of infectious disease testing, the unit must be labeled with

the phrase "Donor Untested"

Which blood group is considered the universal recipient for transfusion of red blood cells

AB

The McLeod phenotype is associated with which of the following antigen systems

Kell

A newborn presents with neonatal alloimmune thrombocytopenia. What is the most likely cause of this condition

maternal antibodies to neonatal platelet antigens

Which of the following is/are considered an accrediting agency(ies) for quality and safety in the blood industry

AABB

If whole blood is stored at a temperature between 2-8C, what is the maximum amount of time you have for use in DNA assays

72 hours

The primary antibody response takes an average of how many days

5-10 days

Which blood component is the most commonly used component for the replacement of multiple coagulation factor deficiencies in bleeding patients

fresh frozen plasma

Which of the following is generally considered equivalent to CMV seronegative RBCs for use in an exchange transfusion to a newborn

fresh RBC less than 7 days old

An error was made in a handwritten blood bank record. Which of the following methods is acceptable for correcting the error to remain in compliance with current good manufacturing practices requirementw

draw a single line through the error so that it can still be read and add you initials next to it

a tech is performing an antibody screen. The reaction is negative when reading at the AHG (anti-human globulin) phase, so the tech then adds Coombs' control cells. Agglutination is now observed. What does this indicate

the negative reaction at AHG phase is valid

A technologist performing an antibody identification in the blood bank has recorded the following results found in the worksheet below. Which antibody(ies) listed below cannot be ruled out

Anti-C, anti-K, anti-E

Which occurrence is a medical error

incompatible blood is transfused into a patient during surgery

Post-natal RhIg is indicated for which of the following mother and neonate pairs

D-positive neonate born to D-negative mother

Donated red cell units contain which substance to stabilize the pH of the unit during storage

sodium biphosphate

Which genotype(s) is/are possible for a mating of an AB mother and an AB father

AB, AA, and BB

In order to prevent a loss of viability in platelet concentrates during storage, the pH must be maintained at or above what level

6.2

Before testing, all cord cells should be thoroughly washed in order to

remove Wharton's jelly

After birth, which of the following is the biggest concern for a newborn infant suffering from hemolytic disease of the fetus and newborn

accumulation of bilirubin

Which of the following fresh frozen plasma ABO types would be suitable for transfusion to an AB negative patient

AB negative and AB positive

Which of the following consequences of severe hemolytic disease of the fetus and newborn (HDFN) is most associated with neonatal death before or shortly after birth

cardiac failure

when a group O patient's serum is not compatible with donor O red blood cells and the auto control is negative, what is the most likely cause

Bombay phenotype

A2B is suspected when a patient's ABO typing has the following results

patient's red cells forward types as AB with anti-A1 present in the patient's serum

The concentration of sodium chloride in an isotonic solution is

0.85%

What is a characteristic of a person who is weak D

demonstrates a reduced number of D antigen sites on the surface of the red cell