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physical conditions include
temp, ph, pressure and concentration
Thermal death time (TDT) (temp)
the shortest amount of time needed to kill microbes at a specific temp
thermal death point (TDP) (temp)
the lowest temp needed to kill all microbes in 10 min
extreme PH can
damage or inhibit microbes
increasing pressure in a autoclave….
raises the boiling point of water allowing steam to reach high temp allowing microbes and endospores to be killed
higher concentrations of antimicrobial agents….
increase the effectiveness of microbial killing
most disinfectants are more active at
higher concentrations