LabEquipment- LAB

Pipets

ARE GLASS OR PLASTIC TUBES, USUALLY OPEN AT BOTH ENDS, WHICH ARE USED TO TRANSFER SPECIFIC AMOUNTS OF LIQUID FROM ONE CONTAINER TO ANOTHER.

True

True or False. Pipets are usually used for volumes between 1 and 100 milliliters.

• TO CONTAIN (TC)

• TO DELIVER (TD)

2 Classifications of pipets based on design

MEASURING OR GRADUATED

DELIVER THE AMOUNT OF LIQUID CONTAINED BETWEEN TWO CALIBRATION MARKS

MEASURING OR GRADUATED

NOT CALIBRATED WITH SUFFICIENT TOLERANCE TO USE IN MEASURING STANDARD OR CONTROL SOLUTIONS.

True

True or False. The frosted band should not be confused with thicker colored rings or colored dots, which are a manufacturer’s code for the maximum volume of the pipette

MEASURING OR GRADUATED

SEROLOGIC, MOHR, BACTERIOLOGIC, BALL, KOLMER, OR KAHN and MICROPIPET are example of

• MEASURING OR GRADUATED based on use

• TRANSFER based on use

False (blow out and self- draining)

True or False. 2 Classifications of pipets based on drainage are measuring/ graduated and volumetric/ transfer.

TRANSFER

VOLUMETRIC, OSTWALD- FOLIN, PASTEUR PIPETS and AUTOMATIC MACROPIPETS OR MICROPIPETS are examples of

• MEASURING OR GRADUATED based on use

• TRANSFER based on use

MOHR PIPETTES

THE GRADUATIONS ON THESE ALWAYS END BEFORE THE TIP

• SEROLOGICAL PIPETTES

• MOHR PIPETTES

False (there should be a frosted band or 2 thin rings)

True or False. Only blow- out a serological pipette if there is no frosted band or two thin rings.

Based on use

Measuring/ graduated and volumetric/ transfer are 2 classifications of

• BASE ON DESIGN

• BASE ON DRAINAGE

• BASE ON USE

SEROLOGICAL PIPETTES

THE GRADUATION MARKS CONTINUE TO THE TIP

• SEROLOGICAL PIPETTES

• MOHR PIPETTES

TO CONTAIN PIPET

CONTAINS A PARTICULAR VOLUME BUT DOES NOT DISPENSE THE EXACT VOLUME

BLOW OUT

HAS A CONTINUOUS ETCHED RING OR 2 SMALL CONTINUOUS RINGS LOCATED NEAR THE TOP OF THE PIPET AND LAST DROP SHOULD BE EXPELLED INTO RECEIVING VESSEL

True

True or False. OSTWALD - FOLIN PIPET is a

• BLOW OUT & TRANSFER PIPET

• FOR VISCOUS FLUIDS

• HAS BULB NEAR THE TIP

VOLUMETRIC PIPET/ TRANSFER PIPET

SHAPED LIKE ROLLING PINS WITH A LARGE BELLY, ONE BLUNT END, THE NECK, AND ONE TAPERING END, THE TIP.

PASTEUR PIPET/ TRANSFER PIPET

DO NOT HAVE CALIBRATION MARKS AND USE TO TRANSFER SOLUTIONS WITHOUT CONSIDERATION OF A SPECIFIC VOLUME

Self- draining

VOLUMETRIC, MOHR are

• Blow-out

• Self- draining

True

True or False. Sahli- Hemoglobin and Lang Levy are rinse out pipet or diluting fluid.

blow-out

SEROLOGIC, OSTWALD FOLIN are

• Blow-out

• Self- draining

To contain pipet/ rinse out pipet (diluting fluid) / Sahli- Hemoglobin, Lang- Levy

A SMALL AMOUNT OF FLUID WILL CLING TO THE INSIDE WALL OF THE PIPET.

SELF-DRAINING

ALLOWS THE CONTENT TO DRAIN BY GRAVITY

True

True or False. VOLUMETRIC PIPET/ TRANSFER PIPET IS USED TO DELIVER A SINGLE SPECIFIC VOLUME OF LIQUID, USUALLY BETWEEN 1 AND 100 ML.

TO DELIVER PIPET

DISPENSE THE AMOUNT OF VOLUME INDICATED AND DESIGNED TO BE DRAIN BY GRAVITY

• AIR DISPLACEMENT

• POSITIVE-DISPLACEMENT

• DISPENSER AND DILUTOR/DISPENSER

Types of Automatic Pipet

False (Vertically)

True or False. MUST BE HELD VERTICALLY AND THE TIP PLACED AGAINST THE SIDE OF THE CONTAINER AND MUST NOT TOUCH THE LIQUID IN IT.

POSITIVE-DISPLACEMENT

OPERATES BY MOVING THE PISTON IN THE PIPET TIP LIKE SYRINGE and DOESN’T REQUIRE DIFFERENT TIPS

• AIR DISPLACEMENT

• POSITIVE-DISPLACEMENT

• DISPENSER AND DILUTOR/DISPENSER

To deliver pipet

A SMALL AMOUNT OF FLUID WILL REMAIN IN THE TIP OF THE PIPETTE AND MEET REQUIREMENTS OF TRANSFER PIPETS

True

True or False. MOHR, SEROLOGIC, VOLUMETRIC TRANSFER PIPETS ARE USED TO DISPENSE THE AMOUNT OF VOLUME INDICATED

False (Positive Displacement)

True or False. Air Displacement Pipet may be required rinsing and blotting between samples.

DISPENSER AND DILUTOR/DISPENSER

OBTAIN THE LIQUID FROM COMMON RESERVOIR AND DISPENSE IT REPEATEDLY.

1. BOTTLE-TOP

2. MOTORIZED

3. HANDHELD

4. ATTACHED TO A DILUTOR

• AIR DISPLACEMENT

• POSITIVE-DISPLACEMENT

• DISPENSER AND DILUTOR/DISPENSER

False (Mohr)

True or False. For Self- draining, the tip of pipet should not be in contact with the accumulating fluid in receiving vessels during drainage except Serologic Pipet

AIR DISPLACEMENT

RELIES ON PISTON FOR SUCTION CREATION TO DRAW THE SAMPLE INTO A DISPOSABLE TIP THAT MUST BE CHANGED FOR USE.

• AIR DISPLACEMENT

• POSITIVE-DISPLACEMENT

• DISPENSER AND DILUTOR/DISPENSER

DISPENSER AND DILUTOR/ AUTOMATIC PIPET

OFTEN COMBINES SAMPLING AND DISPENSING FUNCTIONS

PIPET BULBS

USED TO DRAW LIQUID UP INTO THE PIPETTE

• RUBBER BULB

• PIPET FILLER

• PIPET AID

• PIPET PUMPER

  
  

LIST 4 TYPES OF PIPETTE BULBS

READ PROCEDURE ON USAGE OF PIPET

READ PROCEDURE ON USAGE OF PIPET

True

True or False. The piston of an air displacement pipet does not come in contact with the liquid

Meniscus

CURVATURE IN THE TOP SURFACE OF THE LIQUID.

EYE LEVEL

PIPETTE SHOULD BE

HELD THAT THE CALIBRATION MARK IS AT THE ______ LEVEL.

True

True or False. Calibration is when a glassware or other apparatus used in quantitative measurement is checked to determine its exact volume.

upper meniscus

COLORED/ VISCOUS SOLUTIONS

• upper meniscus

• middle meniscus

• lower meniscus

False (National Bureau of Standards)

Clinical & Laboratory Standards Institute noted that laboratory vessels should be calibrated by weight using distilled water and analytical balance.

VOLUMETRIC FLAKS

CALIBRATED TO HOLD ONE EXACT VOLUME OF LIQUID (TC)

lower meniscus

CLEAR SOLUTIONS

• upper meniscus

• middle meniscus

• lower meniscus

ERLENMEYER FLASK

DESIGNED TO HOLD DIFFERENT VOLUMES RATHER THAN ONE EXACT AMOUNT.

BEAKER

HOLD DIFFERENT VOLUMES RATHER THAN ONE EXACT AMOUNT

True

2 types of beaker: GRIFFIN BEAKERS and BERZELEUS BEAKERS

True

True or False. GRADUATED CYLINDER IS USED TO MEASURE LIQUID WHEN HIGH DEGREE OF ACCURACY IS NOT ESSENTIAL BUT CAN ALSO BE USED TO MEASURE SPECIFIED VOLUME OF LIQUID.

• Liquid-in-glass

• Electronic Thermometer/ Thermistor Probe

• Digital Thermometer

3 Types of Thermometer

Electronic Thermometer/ Thermistor Probe

Type of Thermometer that has advantage of SIZE AND MILLISECOND & RESPONSE TIME and DISADVANTAGE of being EXPENSIVE

• Liquid-in-glass

• Electronic Thermometer/ Thermistor Probe

• Digital Thermometer

• Partial Immersion

• Total Immersion

• Surface Thermometers

List 3 Types of Liquid in Glass Thermometer

Surface Thermometers

FORFLAT SURFACE SUCH AS OVEN OR INCUBATOR

• Partial Immersion

• Total Immersion

• Surface Thermometers

Partial Immersion

USED FOR MEASURING TEMP IN UNITS SUCH AS HEATING BLOCKS AND WATER BATH.

SHOULD BE IMMERSED TO THE PROPER HEIGHT AS INDICATED BY THE CONTINUOUS LINE ETCHED IN THE THERMOMETER

• Partial Immersion

• Total Immersion

• Surface Thermometers

False (Total Immersion)

True or False. Partial Immersion is used for refrigeration application.

Liquid-in-glass

Type of thermometer that is use to color liquid or mercury encased in plastic or glass material with bulb at one end and a graduated stem

• Liquid-in-glass

• Electronic Thermometer/ Thermistor Probe

• Digital Thermometer

False (Liquid- in- Glass)

True or False. Digital Thermometer is usually measures temperature between 20 C to 400 C