1/24
Looks like no tags are added yet.
What is the structure of DNA in non-dividing cells?
Chromatin
What reaction forms a polynucleotide (DNA)?
Condensation reaction
What is the arrangement/structure of DNA?
Anti-parallel double stranded helix
How are chromosomes formed?
Chromatin is wound around histone proteins
How is DNA replication semi-conservative?
One of the original strands is retained and used as a template for the newly synthesised strand
What are the non-coding genes? (STING)
Satellite DNA, Telomeres, Introns, Non-coding RNA, Gene regulatory sequences
What is gene splicing?
The removal of non-coding introns by spliceosomes and the addition of a 5' cap and poly-A tail
What strand is used in transcription?
The antisense strand (template strand)
If the codon on DNA is ATC, what is the corresponding tRNA anticodon?
AUC
What is a polysome?
A cluster of ribosomes on an mRNA molecule
What is gene expression regulated by?
Methylation, acetylation, micro RNA
What is the chemical formula for an amino acid?
NH2 - CHR - COOH
What is the secondary structure of a protein?
The alpha helices and beta pleated sheets formed by hydrogen bonding
What is the tertiary structure of a protein?
The 3D folding with consideration to the functional groups
What is a catabolic reaction?
Breaking down molecules, exothermic
What is an anabolic reaction?
The building of molecules, endothermic
What is a competitive inhibitor?
A molecule that competes with the substrate to bind to the active site of an enzyme
What is a non-competitive inhibitor?
A molecule that binds to the allosteric site of an enzyme, denaturing the active site
What is end-product inhibition?
The final product of a reaction inhibits the first enzyme of a reaction. It is a negative feedback model
What does DNA ligase do?
Anneals nucleotide strands
What are the temperatures involved in PCR?
95, 40, 72
How many times is PCR repeated?
30
What does PCR stand for?
Polymerase Chain Reaction
What is CRISPR?
Clustered Regularly Interspaced Short Palindromic Repeats
What molecules are involved in CRISPR?
Guide RNA, Cas 9