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25 Terms

1
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What is the structure of DNA in non-dividing cells?

Chromatin

2
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What reaction forms a polynucleotide (DNA)?

Condensation reaction

3
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What is the arrangement/structure of DNA?

Anti-parallel double stranded helix

4
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How are chromosomes formed?

Chromatin is wound around histone proteins

5
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How is DNA replication semi-conservative?

One of the original strands is retained and used as a template for the newly synthesised strand

6
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What are the non-coding genes? (STING)

Satellite DNA, Telomeres, Introns, Non-coding RNA, Gene regulatory sequences

7
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What is gene splicing?

The removal of non-coding introns by spliceosomes and the addition of a 5' cap and poly-A tail

8
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What strand is used in transcription?

The antisense strand (template strand)

9
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If the codon on DNA is ATC, what is the corresponding tRNA anticodon?

AUC

10
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What is a polysome?

A cluster of ribosomes on an mRNA molecule

11
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What is gene expression regulated by?

Methylation, acetylation, micro RNA

12
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What is the chemical formula for an amino acid?

NH2 - CHR - COOH\text{NH}_2\text{ - CHR - COOH}

13
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What is the secondary structure of a protein?

The alpha helices and beta pleated sheets formed by hydrogen bonding

14
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What is the tertiary structure of a protein?

The 3D folding with consideration to the functional groups

15
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What is a catabolic reaction?

Breaking down molecules, exothermic

16
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What is an anabolic reaction?

The building of molecules, endothermic

17
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What is a competitive inhibitor?

A molecule that competes with the substrate to bind to the active site of an enzyme

18
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What is a non-competitive inhibitor?

A molecule that binds to the allosteric site of an enzyme, denaturing the active site

19
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What is end-product inhibition?

The final product of a reaction inhibits the first enzyme of a reaction. It is a negative feedback model

20
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What does DNA ligase do?

Anneals nucleotide strands

21
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What are the temperatures involved in PCR?

9595, 4040, 7272

22
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How many times is PCR repeated?

3030

23
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What does PCR stand for?

Polymerase Chain Reaction

24
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What is CRISPR?

Clustered Regularly Interspaced Short Palindromic Repeats

25
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What molecules are involved in CRISPR?

Guide RNA, Cas 9