filter binding assay

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5 Terms

1
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  1. detect binding using


radiolabelled ligand

2
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  1. mix with


unlabelled ligand

3
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  1. add to sample and wait until


equilibrium reached

4
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  1. separate bound and unbound ligands using


nitrocellulose filter through a funnel to trap protein and protein ligand complexes. Leaves only unbound ligand in solution

5
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  1. cut up the filter paper with bound protein and measure


radioactivity using liquid scintillation counter