SCV 213: Lab Fecal Analysis

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6 Terms

1
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What is a direct smear used for?

Detect motile (moving) parasite stages such as protozoan trophozoites

Ex: Giardia lamblia, Tritrichomonis foetus

2
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Simple fecal flotation

Used to detect endoparasitic organisms: eggs or ova, larvae, oocysts, and cysts

Ex: Ascarids (roundworms), hookworms,

3
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What’s the suspension mediums that a simple fecal flotation uses?

  • Sodium Nitrate: general all purpose medium

  • Sheather’s sucrose: Nematodes: Toxocara sp, Ancylostoma sp, Trichuris sp, Capillaria sp
    • Protozoans: Isospora sp.

  • Zinc Sulfate: Giardia lamblia, various lungworms


4
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Fecal Flotation with Centrifugation

Better at detecting ova, cysts & oocysts compared to Simple Fecal Flotation

Ex: Whipworms, Giardia lamblia

5
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Fecal Sedimentation

Used to identify eggs of endoparasites that do not float well in standard flotation media due to high specific gravity & in some cases the presence of an operculum (lid-like cap at end of egg)

  • Ex: Flukes (flatworms) or trematodes (flatworms)


6
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Baermann Technique

Used to identify larvae