lab 2: aseptic technique and isolation strategies

agar

gelatin-like substance used for solid growth media

agar plate

solid medium in a petri dish; lots of surface area

aseptic technique

practices/procedures used to prevent contamination

broth

liquid growth medium for microbes

CFU

colony-forming unit; one cell or a group of cells acting as one unit

colony

many cells visible to the naked eye resulting from a single cfu; all genetically identical, pure culture

contamination

a microbe that's out of place (where it shouldn't be)

deep

solid medium in a culture tube left upright; growth in media; more surface area

incinerator (bactincinerator)

electric heat used to sterilize inoculating tools

inoculating (transfer) loop

collects media or broth and transfers it; wire and loop on other end

inoculating (transfer) needle

stabbing deeps, minimizing damage to medium; wire and no loop

isolation

to create a pure culture from a mixed population

micropipette

used to transfer small amounts of liquid

pure culture

only one type of species is present; microbes are genetically same

slant

solid media in culture tube left slanted; growth on surface

inoculation

transferring microbes to a new location

growth media

material used to grow microbes