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What is the Cell Theory?
Al organisms are made from cells
Cells are the basic building blocks for life
All cells come from pre-existing cells that have multiplied
What is a prokaryote?
No nucleus and DNA floats around in the cytoplasm
Example of prokaryote
Bacteria
What is a eukaryote?
Has a defined nucleus and DNA is packed away in the membrane
Example of eukaryote?
People, trees, animals
What is the cell membrane?
Outer wall of a cell
What does the cell membrane do?
allows materials in and out of the cell
What is the nucleus?
Stores DNA that is usually found in Chromatin
What cells are the nucleus found in?
Eukaryote
What are nuclear pores?
Allow things in/out of the nucleus
What is the nuclear envelope?
Protective barrier
What is the nucleolus?
produces ribosomes and rRNA produces instructions for DNA
Where is the nucleolus?
Nucleus
What cells is the nucleolus found in?
Eukaryote
What is the smooth ER?
Makes lipids
An example of lipid
Cholesterol, phospholipids, steroid hormones
Smooth ER can sometimes…
break down toxins and drugs and aid in muscle contraction
What is the Rough ER?
Makes proteins with ribosomes and has ribosomes attached
What is the Golgi Aparatus?
receives and modifies rough ER products
Where is the Golgi Aparatus?
Near the nucleus and the ER
What are Lysosomes?
Digest food and waste and destroy bacteria
Where are lysosomes?
freely within the cytoplasm
What cells have lysosomes?
Animal cells
What is a vacuole?
Storage vesicle
Example of vacuole?
water storage, pigment storage
What is a contractile vacuole
pumps out water in some small eukaryotes so they don’t swell and burst
What is a vacuole in plants?
Stores water, waste, digestive enzymes, and helps maintain pressure in the cell
Where is the vacuole in plants?
Large central vacuole
What is a vesicle?
Transport material throughout the cell
What can the vesicle do?
fuse with or pinch off other membrane-bound organelles
What is the structure of a vesicle?
Small spheres of membrane
What cells are vesicles found in?
Eukaryotes
What is the mitochondria?
Converts sugar energy in ATP
What cells have mitochondria?
Animal and plants
What is the structure of mitochondria?
Outer membrane, inner membrane, and mitochondrial matrix
What is the inner membrane of the mitochondria?
Folded to increase surface area so more ATP can be made
What is mitochondrial matrix?
Contains mitochondrial DNA and ribosomes
What is cellular respiration?
creates energy but breaking down sugar
What are Chloroplasts?
Converts light energy into glucose
What is the Chloroplasts structure?
DNA, ribosomes, double membrane
What are Thylakoids?
Stacks of granum in the chloroplasts
What are Stroma?
Liquid surrounding thylakoids
What cells have chloroplasts?
plant cells
What is photosynthesis?
Light + Water + Co2 = sugar + oxygen
What is the cell wall?
Provides protection for the cell from damage and support
Where is the cell wall found?
outside of cell membrane
What cells have cell walls?
Plant cells
What organelles do plants have?
Large central vacuole, cell wall, chloroplasts
What organelles do animals have?
lysosomes, small vacuoles
What organelles do animals and plants both have?
Nucleus, cell membrane, mitochondria, ribosomes
What is the endomembrane system?
manufactures and distributes cells and divides cells into compartments
What organelles are involved in the endomembrane system?
Nuclear envelope, ER, Golgi Apparatus, Lysosomes, Vacuoles, Plasma Membrane
Organelles in the endomembrane system are…
Some are connected, others are linked by transfer of vesicles
What is endosymbiosis?
Theory that describes the origen of the mitochondria and the chloroplasts
What did the theory state?
There was once a large eukaryote cells that had a symbiotic relationship with a smaller prokaryote (primitive eukaryote + aerobic prokaryote) and the eukaryote took in the prokaryote to make Mitochondria
How was the chloroplasts made?
Eukaryote absorbing cyanobacteria to make chloroplasts
What is a light microscope?
uses light to see an image
What is a light microscope often called?
a compound microscope because it has 2 lenses (objective lens and eyepiece/ocular lens)
What is the eyepiece?
Where you look in to see the specimen
How much does the eyepiece magnify by?
10x
What is the objective lens?
Has 3 lenses (scanning, low power, and high power)
How much does scanning lens magnify by?
4x
How much does low power lens magnify by?
10x
How much does high power lens magnify by?
40x
How can you get the total magnification?
By multiplying the eyepiece and objective lens
What is the frame?
The arm of the microscope
What is the stage?
where the specimen is placed
What are stage clips?
secure the slide to the stage
What is stage controls?
Moves the stage side to side
What is an Iris Diaphragm?
adjusts the level of light that projects onto a specimen
What is course focus?
Focuses on larger objects
What is fine focus?
focuses on smaller objects
What focus should you not use at 400x and why?
Course focus because it can break the lens
What is illumination?
Light that passes through the condenser lens so you can see the specimen
What is the field of view?
The circular field that you see through the eyepiece
What happens to the field of view as you increase magnification?
It gets smaller
How are the lengths of microscopic images expressed?
In millimeters or micrometers
What is a millimeter?
10-3 of a meter
What is a micrometer?
10-6 of a meter
How can you find micrometers from millimeters?
Millimeters x 103 = micrometers
What is the depth of field?
How focused or unfocused your specimen is
What is compartmentalization in a cell?
The cell is divided up into groups composed of single or double membranes
What is the most important compartment in the cell?
Nucleus
What happen to the enzymes in compartmentalization?
Enzymes are less spread out making reactions for efficient
What happens to the damaged areas in compartmentalization?
Damaged areas can be kept together so it doesn’t damage the overall cell