Parasitology Lab Practical 1

• human pathogen?

• where is this stage found?

Giardia duodenalis, Trophozoite

-CAN be human pathogen

-found in small instestine

• where is this stage found?

Giardia duodenalis, Cyst

-found in feces

-infective stage!

where would you find this?

how many flagella does it have?

Trichomonas vaginalis, trophozoite

-found in urethra or prostate/ vagina

-5 flagella

-is it pathogenic?

-where is found?

Entamoeba histolytica, troph

-round nucleus; endosome centered perfectly within nucleus

-pseudopod

-pathogenic!

-large instestine

- 4 nuclei

- chromatoid bar

-where is it found?

Entamoeba histolytica, cyst

-endosome NOT necessarily centered in nuclei

-infective stage!

-found in feces

• where is it

Entamoeba coli, troph

-cytoplasm has engulfed debris

-pseudopod

-endosome NOT centered in nucleus

-intestine

is this pathogenic?

Entamoeba coli, cyst

-8 nuclei

-grey and faint

NOT pathogenic

-is it big or small?

Endolimax nana, troph

-single nucleus

-large, blot-shaped endosome

-small body

is pathogenic?

Iodamoeba buetschlii, cyst

NOT pathogenic

-big or small?

Balantioides coli, troph

-very large (60 um)

-cilia!! outer border

-large dark nucleus, sausage shaped (15 um)

Balantioides coli, pathology

-parasites per RBC?

Plasmodium vivax, troph

-Schuffner’s dots

-signet ring

-ONE parasite per RBC!

Plasmodium vivax, schizont

-blood smear

-multiple nuclei

-dark waste sometimes visible (hemozoin granules)

-infective to what?

Plasmodium vivax, macrogametocyte

-blood smear

-infectivie to mosquitos!

-fills majority of RBC

-hemozoin granules (waste)

-parasites per RBC?

Plasmodium falciparum, troph

-MULTIPLE parasites in one RBC!

diff b/w Plasmodium falciparum (troph) and Plasmodium vivax (troph) ?

micro vs macro

• where and in which host does it appear?

• what is this process called?

Plasmodium falciparum, microgametocyte

macro= banana

micro= sausage

-nucleus appears as dot clusters in center

• stomach of female mosquito

• gametogony

-life cycle stage following this?

Plasmodium vivax, oocyst

-stage= sporogony

infective to what??

Plasmodium vivax, sporozoites

-infective to humans!!

sporogonoy . merogony. all that /// in order!

zygote/ OOCYST→ (Sporogony) —> sporozoites → (merogony) → merozoites

parasitic to what?

Haemoproteus columbae, troph

-parasitic to birds/reptiles (invertebrate)

Eimeria stiedae, schizont

-darker shaded nucleus bunched at end

Eimeria stiedae, macrogametocyte

-female s3xual developmental stage

Eimeria stiedae, microgametocyte

-male s3xual reproductive stage

Eimeria stiedae, unsporulated oocyst

-must be found in liver (NOT in feces yet)

Eimeria stiedae, sporulated oocyst

-fecal smear

-micropyle (aka pore) on one end

-sporozoites in oocyst

big or small?

Toxoplasma gondii, tachyzoites

-super small

-mandarin shaped!

asexual or sexual?

hallmark of which phase?

what life cycle stage is within ?

Toxoplasma gondii, zoitocysts w/ bradyzoites inside!

-asexual!

-hallmark of the extraintestinal phase

-bradyzoites

-contain what life cycle stage

Sarcocystis, zoitocysts

-resemble grains of rice

-Zoitocysts contain the asexual life cycle stages known as bradyzoites

what type of parasite?

Naegleria sp. , amoeboid troph

-facultative parasite

small or big?

Leishmania donovani, amastigote

-hamster spleen

-tiny!!

-short flagellum

Leishmania donovani, promastigote

-kinetoplast near flagellum (long)

Trypanosoma lewisi, trypomastigote

-kinetoplast posterior to nucleus (flagella @ top!!)

victim (DH)= mice & rats

IH= rat fleas

Trypanosoma brucei rhodesiense, trypomastigote

diff between Trypanosoma brucei and lewisi

T.B. gamiense or rhodesiense or brucei ; DH and vector of each?

Tsetse fly for all!

T.B. gamiense & rhodesiense= humans

T.B. brucei = animals

Trypanosoma cruzi, trypomastigote

-South america!

diff b/w T. cruzi and T. brucei

Trypanosoma cruzi, amastigote

-infected heart mucles; concentrated pockets = pseudocysts

Trypanosoma equiperdum, trypomastigote

-direct transmission via s3x in horses, donkeys, and mules

big or small

Trypanosoma rotatorium, trypomastigote

-large!

-extracellular!

IH= leeches

Dh= frogs

Leishmania donovani, promastigote

-kinetoplast anterior to nucleus!

-host = sandfly

Leishmania donovani, amastigote

-nucleus and kinetoplast most visible (look for 2 dots)

-super tiny (3 um)

-amastigote= nucleus and kinetoplast close together

Leishmania tropica, promastigote

Triatoma sp, (Kissing bug)

Lutzomyia sp. (sandfly)