Nematodes

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What is the specimen of choice for nematode?

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1

What is the specimen of choice for nematode?

Stool

  • eggs (most common)

  • larva

  • rarely adults

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2

Which larval stage is usually the infective stage for nematodes?

Filariform larvae

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3

What two species are the hookworms?

Ancylostoma duodenale Necator americanus

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4

How is the method of infection different for hookworms and Strongyloides compared to other nematodes?

Penetration of skin via filariform larvae

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5

Besides the intestines, named two alternative body sites for infection and the parasite(s) associated with each.

Lungs

  • Ascaris lumbricodes

  • Hookworms

  • Strongyloides

Tissue

  • Trichinella spiralis

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6

Common name for Enterobius vermicularis?

Pinworm

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7

Infective stage of pinworm/E. vermicularis

Egg - ingestion (dirty hands, toys, bedding, etc.)

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8

Diagnostic stage of pinworm/E. vermicularis

Eggs, larvae, adults in stool

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9

What is pruritis and which parasite is it associated with?

Intense anal itching E. vermicularis

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10

Laboratory diagnosis of E. vermicularis

Wet mount Scotch tape test

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11

Common name for Trichuris trichiura

Whipworm

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12

Infective stage of whipworm/T. trichiura

Eggs - ingestion

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13

In heavy whipworm infection, what rectal symptoms might manifest?

Rectal prolapse

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14

Laboratory diagnosis of T. trichiura?

Wet mount

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15

Common name for Ascaris lumbricoides

Giant intestinal worm

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16

Infective stage of A. lumbricoides

Eggs - ingestion

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17

Diagnostic stage of A. lumbricoides

Eggs in feces Larvae in sputum

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18

What alternative body site does A. lumbricoides infects?

Lungs

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19

Symptoms of A. lumbricoides infection

Intestinal

  • usually asymptomatic unless the load is heave

  • Abdominal pain

  • Vomiting, nausea

Lungs

  • Pneumonia

  • Asthma

  • Eosinophilia

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20

Laboratory diagnosis of A. lumbricoides

Wet mount Sputum

ELISA also available

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21

Common name for Ancylostoma duodenale

Old World hookworm

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22

Common name for Necator americanus

New World hookworm

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23

Infective stage of hookworm

Filariform larvae - penetration of skin

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24

Diagnostic stage of hookworm

Eggs in feces

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25

What alternative body site can hookworm infects?

Lungs

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26

How to differentiate btw. Ancylostoma and Necator?

Eggs are identical

Ancylostoma - teeth

Necator - cutting blades

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27

Symptoms of hookworm

Pulmonary issues Vomit, nausea, diarrhea Iron deficiency anemia Larval dermatitis (serpiginous tunnels)

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28

What causes cutaneous larval migrans?

Ancyclostoma & Necator

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29

What causes creeping eruption?

Ancyclostoma & Nector

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30

Laboratory diagnosis of hookworm

Wet mount

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31

Common name for Strongyloides stercoralis

Threadworm

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32

Infective stage of Strongyloides

Filariform larvae - penetration of skin

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33

Diagnostic stage of Strongyloides

Rhabiditiform larvae in feces

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34

What alternative body site can Strongyloides infects?

Lungs

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35

Symptoms of Strongyloides

Pulmonary issues Vomit, nausea, diarrhea Erythema Can be fatal in IC patients

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36

Laboratory diagnosis of Strongyloides

Wet mount Sputum or bronchial samples Enterotest (string test) Antibody detection

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37

How to acquire Trichinella spiralis?

Ingestion of undercooked meat (pork, bear, walrus)

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38

Infective stage of Trichinella

Encysted larva - ingestion

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39

Diagnostic stage of Trichinella

Encysted larva in tissues/muscles

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40

Symptoms of Trichinella

Intestinal issues Fever, eosinophilia Muscle pain Eye edema

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41

Laboratory diagnosis of Trichinella

Muscle biopsy Serology

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42

What can cause visceral and ocular larval migrans?

Toxocara cati/canis

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43

Infective stage of Toxocara

Eggs - ingestion

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44

Diagnostic stage of Toxocara

Larvae - biopsy

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45

How to acquire Toxocara?

Ingestion of eggs from cat/dog feces

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46

Symptoms of Toxocara

Generally not severe Fatigue, weight loss Ocular problem Eosinophilia

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47

Laboratory diagnosis of Toxocara

Biopsy Serology

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