CP 1 benedict's test for reducing sugars unit 3

what does benedict’s reagent test for

reducing sugars

how do the results of the benedict’s test indicate different concentrations of reducing sugar

blue : negative

as the colours got closer to brick red (green → yellow → orange → red) the reducing sugar concentrations increase

what are the controlled variables when using benedict’s reagent to estimate reducing sugar concentrations

volume of benedict’s reagent

volume of sample added

time left in water bath

what does iodine solution test for

starch

what is the observation for a positive iodine test

the solution turns from brown to blue black

outline the procedure to find the concentration of an unknown reducing sugar solution

carry out benedict’s tests for a range of standard reducing sugar solutions to produce colour standards carry out a benedict’s test on the unknown solution (using the same volume of solution and length of time in the water bath) and compare the resulting colour