Study Guide for BIOL 2421 Lab Practicum (Subject to Change)

Gram Stain must be completed in __________ minutes.

20

In the Gram Stain test, students are provided with a numbered heat-fixed smear of an unknown __________.

bacterium

The possible bacteria for the Gram Stain include Staphylococcus epidermidis and __________.

Escherichia coli

Shape of Staphylococcus epidermidis = __________.

coccus/cocci

Arrangement of Staphylococcus epidermidis = __________.

staphylococcus/staphylococci

Gram stain result for Staphylococcus epidermidis = __________.

Gram positive

Shape of Escherichia coli = __________.

bacillus/bacilli

Arrangement of Escherichia coli = __________.

individual or scattered

Gram stain result for Escherichia coli = __________.

Gram negative

Instructors will observe and grade the quality of your Gram Stain after it is focused properly under the __________.

microscope

Recording observations for the Gram Stain includes shape, arrangement, and __________ result.

Gram stain

A streak plate is not __________.

timed

Students must perform the streak plate while using proper __________ technique.

aseptic

Positive for H2 S production shows a __________ coloration in the butt of the tube.

black

Kligler's Iron Agar results are indicated by RED = __________ and YELLOW = __________.

negative, positive

To determine if a culture is mixed or pure, students will need to observe an __________ plate.

agar

In the Acid-fast stain, hot pink indicates __________ bacteria.

acid-fast

For capsule staining, a colorless capsule indicates the presence of a __________.

capsule

The objective of evaluating a Kirby-Bauer plate is to determine the __________ of inhibition diameter.

zone

To analyze MPN results, students must determine the number of tubes positive for acid and __________ production.

gas