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Heterologous probe
DNA or RNA sequence from a different organism or gene used to find a related gene by sequence similarity
Heterologous probe purpose
cross species gene id
guessmer
A synthetic DNA probe made from a known amino acid sequence when the gene’s DNA sequence is unknown.
mutant complementation
Technique where a mutant organism (missing a gene function) is transformed with a library of DNA to find a clone that restores the missing function.
restriction mapping
A method to determine the order and distances between restriction enzyme sites in a DNA molecule.
southern blot
Technique to detect specific DNA sequences within a complex mixture.
northern blot
Same idea as Southern blot, but detects RNA (usually mRNA).