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7 Terms

1
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Heterologous probe

DNA or RNA sequence from a different organism or gene used to find a related gene by sequence similarity

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Heterologous probe purpose

cross species gene id

3
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guessmer

synthetic DNA probe made from a known amino acid sequence when the gene’s DNA sequence is unknown.

4
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mutant complementation 

Technique where a mutant organism (missing a gene function) is transformed with a library of DNA to find a clone that restores the missing function.

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restriction mapping

A method to determine the order and distances between restriction enzyme sites in a DNA molecule.

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southern blot

Technique to detect specific DNA sequences within a complex mixture.

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northern blot

Same idea as Southern blot, but detects RNA (usually mRNA).