Homework questions

Use this model of a eukaryotic primary transcript to answer the following question. Assuming that: E = exon, I = intron and UTR = untranslated region.

 

5′- UTR E1 I1 E2 I2 E3 I3 E4 UTR -3′

 

If the primary transcript is processed and transported to the cytosol, which of the following molecules best represents the mRNA that will be produced?

 

 

 

5′- UTR I1 I2 I3 UTR -3′

5′- E1 E2 E3 E4 -3′:

5′- UTR E1 E2 E3 E4 UTR -3′

5′- E1 I1 E2 I2 E3 I3 E4 -3′

5′- UTR E1 E2 E3 E4 UTR -3′

Which of the following processes correctly describes alternative RNA splicing?

It is a mechanism for increasing the rate of translation.

It can allow the production of proteins of different sizes and functions from a single coding gene.

It can allow the production of similar proteins from different RNAs.

It increases the rate of transcription.

It can allow the production of proteins of different sizes and functions from a single coding gene.

Which of the following statements best describes the significance of the TATA box in the promoters of eukaryotes?

It is the recognition site for the binding of a specific transcription factor.

It sets the reading frame of the mRNA during translation.

It is the recognition site for ribosomal binding during translation.

It is the recognition site for ribosomal binding during transcription.

It is the recognition site for the binding of a specific transcription factor.

Which answer correctly compares the primary transcript in the nucleus of a eukaryotic cell with the functional mRNA?

the primary transcript is the same size as the mRNA

the primary transcript is larger than the mRNA

the primary transcript is smaller than the mRNA

both the primary transcript and mRNA contain introns

the primary transcript is larger than the mRNA

Match the question to its answer

Transcription in eukaryotes requires which molecules in addition to RNA polymerase?

transcription factors

In eukaryotes, there are several different types of RNA polymerase. Which type is involved in transcription of mRNA for a protein?

RNA polymerase II

Which process occurs as part of transcription?

RNA is synthesized

During elongation, nucleotides are added in which direction?

5' to 3' direction

Why is it important to identify the promoter region first before the transcription process can start?

It is where the RNA polymerase binds to so the reading of the bases can start

It provides the necessary enzyme needed for the transcription to proceed

It removes the cap that regulates the transcription process

It provides the template which will serve as the pattern to be transcribed

It is where the RNA polymerase binds to so the reading of the bases can start

Which of the following statements about RNA processing is FALSE? 

Introns are removed before mRNA leaves the nucleus

The 5' end will be capped

Poly-A tail will be added at the 3' end

The spliceosomes remove the exon in pre-mRNA

The spliceosomes remove the exon in pre-mRNA

A particular triplet of bases in the template strand of DNA is 5′-AGT-3′. What would be the corresponding codon for the mRNA that is transcribed?

3′-UCA-5′

3′-ACU-5′

5′-AGT-3′

5′-UCA-3′

3′-UCA-5′

Which of the following is most likely to occur if a researcher removes the 5′ cap and poly-A tail of an mRNA and inserts the mRNA molecule into a eukaryotic cell?

The mRNA will be quickly converted into a ribosomal subunit.

The cell will add a new poly-A tail to the mRNA.

The mRNA will attaches to a ribosome and be translated, but more slowly.

The molecule will be degraded by enzymes.

The molecule will be degraded by enzymes.

Below is a segment of DNA from within a gene. The beginning of the gene has already been transcribed. Which of the following would be the mRNA sequence transcribed from this DNA segment?

 

3'-CCGUGCGCUUCA-5'

5'-GGCACGCGAAGU-3'

5'-UGAAGCGCACGG-3'

5'-CCGUGCGCUUCA-3'

5'-GGCACGCGAAGU-3'