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Vacuole
A fluid-filled organelle found in the cytoplasm of most plant cells. A plant cell usually has one large vacuole that occupies much of its volume.

Tonoplast
The selectively permeable, single membrane surrounding a vacuole.
Cell sap
The liquid inside a vacuole, consisting of water, mineral salts, sugars and amino acids.
Functions of vacuoles
Store water and other substances, help with digestion and excretion of cellular waste, and maintain the shape of plant cells.
Turgor pressure
The outward pressure produced when a water-filled vacuole pushes the cell membrane against the cell wall.