RP - Using a Light Microscope

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4 Terms

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What is Magnification?

How many times larger the image is

2
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What is Resolution?

How easy it is to distinguish between two points that are close together

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Using The Microscope Method (5)

  1. Start with the lowest magnification objective lens. Provides the widest field of view to know which area to increase the magnification on.


  1. Use the coarse focus knob to bring the slide into general focus.


  1. Use the fine focus knob to sharpen the image.


  1. Switch to a higher magnification objective lens if needed and refocus using only the fine focus knob.


  1. Calculate total magnification:
    Total Magnification = Eyepiece Lens magnification × Objective Lens magnification


4
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Name the pieces of a microscope