Microbiology Practical Exam

Specimens/Diag:

CSF - Menigitis Purulenta

Puncture sinus - sinuitis maxillaris

Pus secretion - abcessus submandibularis

Stain: Gram pos cocci in clusters

Media:

1. Blood agar plate

2. MRSA screen agar

iDENTIfy:

1. golden yellow pigment of colonies on BAP

2. B-hemolysis on BAP (outside)

3. PlasmaCoagulase TUBE test - coagulase +

4. MANNITOL AGAR TUBE TEST

purp - yelo = facultative anerobes

mannitol deg - anerobic or aerobic

Staphylococcus Aures

Specimen/Diag:

CSF- Meningitis Purulents

Throat specimen - Angina LACUnaris

Pus sectrection - pHELGmona manis

Stain - Gram +ve cocci in chains

Media - Blood Agar plate

Identification/Diag:

1. b-hemolysis on BAP

2. small grey colonies

3. Bacitrin paper disc test - group A streptococci sensitive

(Str pyrogens - also sensitive )

4. CAMP Test - positive for group B steptococci (+ s.agalactiae)

Streptococcus B-hemolyticus

Specimen/Diagnosis:

CSF - Meningitis OTOgenica

Ear Specimen - Otitis media (infection of mid ear)

Stain - Gram +ve cocci in PAIRS

- Klett stain - test for capsule

Media - Blood Agar Plate

Identification/Diagnosis:

1. Alpha hemolysis on bap - discoloration around colonies

2. Paper disc test - sensitive to optochin

3. Inulin test - positive

4. sensitive to bile

5. sepsis in mice

Streptococcus Pneumonia

Specimen/Diagnosis

Sputum - Tuberculosis pulmonis

Stain:

Ziehl-Neelson - acid fast bacteria

- bright RED colonies

Media:

Lowenstein-Jenson

Identification/Diagnosis:

1. Rough yello/crem colonies post 40days for cultvation

2.PCR test on BAP -6HR

3.TB spot test - t cells activate against tb

4. Mantoux skin test

5. BCG vaccine - live attenuation

Mycobacterium Tuberculosis

Specimen/Diagnisos:

Throat - pSEUDOMEMbranous Angina

Stain:

Neisser staining- blue/black

arranged in Chinese letters/palasides

Media:

1.Loeffler

2. Klauberg

3 types of colonies:

gravis = Rough

mitis = Smooth

Intermedius= S+R

Identification/Diag:

1.API Coryne

2. detection of exotoxins:

in-viva - neutralisation test

in-vitro-Elech's ppt test

3. glucose +ve, maltose +ve

4. vaccine anatoxin (DTP)

Corynebacterium DIPHtherIAE

Specimen/Diagnosis:

Nasopharyngeal Swabs - Wh00ping cough

Stain:

Gram -ve , RED COCCI

Media:

1. Choco agar - factor 5 & 10

2. Blood agar - S.aures - provides factors 5+ 10

Identification/Diag:

1. Translucent colonies seen

2. Satellite phenom

3.Slide agglutination test - Deteot type B

Bordella Pertusis

Specimen/Diagnosis:

CSF- Meningitis Purulenta

Stain:

Gram -ve, coccobacilli, pleoMORPHIC

Media:

1.Choco agar

2.Blood agar - SATELLITE PHENOM - S.aures(trans colonies) provide factor 5+10

3. Levinthal agar in pouch

4. Oxidase test - +ve (smallest test tube)

5. Thioglycolate broth (big test tube )

Identification/Diag:

Satellite Phenom (3 lines - TRINITY)

Slide agglutination test - To detect type B

Positive Oxidase test

Hemophillus Influenza

Specimen/Diagnosis:

Urethral secretion- Obs GoNorrHOeae

Infections in genitals,rectum,throat

Stain:

Loeffler (methylene blue) - IntrAcytoPlasmic Diplococci, Hyperchromic

dark blue kidney shaped

Media:

Thayer-Martin

Identification/Diag:

1. Donut shaped diplococci

2. Glucose +ve

3.Catalase +ve

4. Oxidase +ve

NEISSERIA GONORRHOEAE

Specimen/Diagnosis:

CSF- Meningitidis EPIDEMICA

Stain: Loeffler stain (methylene blue)

intracellulary hyperchromatic diplococci

Media:

1.Choco Agar - transparent colonies

2.Blood Agar Plate - grey colonies

3. LevinTHAl agar in pouch

Identification/Diag:

1.Oxidase test +ve

2.Glucose test +ve

3. Maltose test +ve

4. Slide agglutination test

NeissERIa mENINGTIDIS

Specimen/Diagnosis:

Wound secrection - Telanospasim (muscle spasm ,cramping)

Stain:

Gram +ve - drumstick apperance

Media:

1.Robertson's - cooked meat broths

2.Thioglycolate broth

3.Blood Agar Plate

Identification/Diag:

Roberts - produce gas in medium - burns meat black

BAP- produce a fine film of growth

C.tetani is HEMOlytic

Indole production (_+)-contains trytophan (turns red )

Clostridium Tetany

Specimen/Diagnosis:

Wound secretion - Gas gangrene

Stain:

Gram +ve - large rods

Media:

1.Zeissler blood agar

2.Thioglycolate broth

3. Wilson-Blair agar + anaerobic atms

Identification/Diag:

1. Milk coagulation test (blue-pink)

2. Wilson-blair agar black colonies

3.Zeissler agar in ANAEROBIC pouch

4. Thioglycolate broth-used as red agent

Clostridium Perfinges

Specimen & Diagnosis:

Pustula secretion - Pustula MALIGna (cutaneous anthrax

Stain:

Klett for capsule

blue rods in chains surronded by PINK capsule

Media

1.Simple broth

2.Simple agar

Identificaton/Vacccine:

1.Cotton-like sediment in broth

2. R-colonies = Medusa head

3. AsCOLi - ring ppt test

Bacillus Anthracis

Stain:

Spores are Moller stained

blue rod shaped bacteria with central non-deforming red spores

Spores

Specimen/Diagnosis:

Faeces/vomiting material - Cholera disease

Stain:

Gram staining -ve

Media:

Thiosulfate Citrate Bile sucrose agar

Identification/Vaccine:

*Yellow colonies on green agar

-Oxidase +ve

-Nitrate +ve

-Carboxylase +ve

-String test -forms a thread-like mucoid string +ve

Vibrio Chloera

Specimen/Diagnosis:

1.Lymph node biopsy Plague Disease

2. Sputum

Stain:

Gram -ve

Media:

Blood Agar

Identification/Vaccine:

Grey white transulucent colonies (24h)

-Catalse +ve

-Nitrate reduction +ve

-Has methyl red

-Maltose

-Mannitol +ve

YerSINia Pestis

Specimen/diagnosis:

LACTOSE FERMENTERS

- Motilie - E.coli

-non motile - klebsiella

NON-LACTOSE Fermenters

-Motile -Proteus, Salmonella

-Non-motile - Shigella

Stain:

Gram -ve - rod shaped

Media:

Kligler-iron agar

EMG agar

Identification/Vaccine:

Black colonies with green metallic sheet.

Catalase +ve

blood agar ---Swarming

Enteric bacteria

Specimen/Diagnosis:

mouth specimen - SOOR (candidiasis) ,

ORAL THRUSH

Staining:

1. Loeffler (methylene) blue

2. Oval shaped fungi

Media:

Candida Chrom AGAR

Identification/Vaccine:

1. Green colonies on chromogenic media

2. API 20C Aux-biochemical identification)

Candida Albicans

Specimen/Diag:

Hemoculture -EndocardIDitis LeuTA

Stain:

Gram +ve cocci in chains

Media:

1. Bottle for hemoculture

2. Blood Agar Plate

Identification/Vaccine:

1.Alpha hemolytic colonies on blood agar plate (green)

2.Optocin -ve test

3.Inulin -ve test - blue

Streptococcus alpha hemolyticus

Specimen/Diagnosis:

Hemoculture - TYPHOID FEVURRRR

Stain:

Gram -ve, medium chains

Media:

1. Bottle for hemoculture

2.Levin Agar

3. DCA

Identification/Vaccine:

Levin - colourless colonies on agar - lactose -ve

DCA - colourless colonies with black cEntre on DCA

Kliger test tube- titled red, black, yellow - BLACK represents hydrogen sulfide

H2S positive

Salmonella Typhi

Specimen & Diagnosis:

Wound secretion - combustion 2nd Grade

Stain:

Gram -ve rods

Media:

1. Glucose broth

2. BAP

3. Simple Agar

4. Antibiogramme

Identification/Vaccines:

1.Glucose broth (test t) -growth in surface/darker

2. Blood agar - b hemolytic ROUGH colonies

3. Oxidase tube test (small) - +ve

4. Simple Agar - green pigmented colonies

4. linden tree colonies

Pseudomonas Aeruginosa

Specimes/Diagnosis:

CSF - Meningitis purulenta

Urine - Cystopyelitis Acuta

Stain:

Gram -ve rods

Media:

Levin agar

DCA

Identification/Vaccine:

1. Levin agar - lactose +ve (green)

2. Kleiger medium tube (yellow)

glucose +ve

gas +ve

H2S -ve

3. DCA - bile salts pink colonies

EscheRICHia COLI

Specimen & Diagnosis:

Urine- Pyelonephritis acuta

Stain:

Gram -ve rods

mEDIA:

Levin agar

Simple agar

Identification/Vaccine:

1. Lavinar agar - lac -ve and colorless

2. HS2 +VE

3. DCA colonies with black cetre

4. Urease +ve test (red)

5. Simple agar - swarming phenomenon (wAVEZ)

6. Klger medium -test tube red

Proteus Spp

Specimen & Diagnosis

Stools- Salmonella food poisioning

Stains:

Gram -ve rods

Media:

Levin Aga r

DCA

Selinite broth

Identification/Vaccine:

1. Levin agar - lac -ve

2. Klieger tube - red,blackyellow

gluc+,gas both,H2S -

3. DCA -lactose -ve col , black centres

4. slide agglut test

Samonella Spp

Specimen & Diagnosis

Stools - Dystery - intestinal inflammation

Stain:\Gram -ve rods \

Media:

Levin agar

DCA

Selenite broth

Identification/Vaccine:

1. Levin agar- lactose -ve colonies

2. DCA lac negative

3. Kligler test tube

glu +ve

gas -ve

H2S -ve

4. Slide agg test

Shigella Spp

Specimen & Diagnosis

Pustlum Maligna Section - Pusula Maligna

Stain:

Klett for capsule

Media:

1. Simple broth

2. Simple agar

Identification/Vaccine:

1. Simple broth test tube (screw top) - cotton like sediment

2. Simple agar - rough colonies 'medusa head'

3. Ascoli ring ppt test

Bacillus Anthracis