biol 231 lab 5 quiz

what is often the first method used in studies investigating the involvement of a particular protein in a cellular process?

protein quantitation

How can the number of protein moelcules in a volume of substance be measured in protein quantitation?

absorbance of light

what does Beer’s law state?

that the absorbance of light of a particular wavelength by a solute is directly proportional to the concentration of the solute in a solution

what is the function of the dye Coomassie Blue?

binds to side chains of certain amino acids including arginine, tryptophan, tyrosine and histidine and produces a blue color

what is the intensity of the dye Coomassie Blue correlated with?

the amount of protein in the sample

the use of the dye Coomassie Blue to measure protein concentration was first described in 1976 by whom?

M. Bradford

in this laboratory, what will we be using the Bradford assay to do?

create a standard curve using absorbance data from sample of proteins of known concentrations

what is a spectrophotometer and what is its function?

an instrument with a light source that generates specific wavelengths and a detector that reads the amount of light either transmitted or absorbed by a sample that is placed in the light path

in the Bradford assay, the spectrophotometer is set to read at ____ nm since the park absorbance of Coomassie Blue once bund to proteins is at 595 nm

595