Untitled Flashcard Set

LB Agar

Also known as lysogeny broth, it is a multi-purpose media capable of growing a wide variety of microorganisms, particularly E. coli.

Trypticase Soy Agar (TSA)

A multi-purpose media similar to LB agar, used for growing a variety of microorganisms, and serves as a base for formulating specialized media.

Blood Agar (BAP)

A derivative of TSA with added mammalian blood to promote growth of fastidious microorganisms; used to detect hemolytic activity.

Alpha Hemolysis

Partial lysis of red blood cells on blood agar, resulting in greenish-brown color colonies.

Beta Hemolysis

Complete lysis of red blood cells on blood agar, resulting in a clear zone around colonies.

Gamma Hemolysis

Absence of hemolytic activity on blood agar, typically resulting in white/tan colonies.

Columbia CNA Agar

An enriched, selective, and differential media that suppresses Gram-negative bacteria growth for isolating Gram-positive microbes.

Chocolate Agar (CHOC)

An enriched, non-selective derivative of blood agar with lysed red blood cells that provides growth factors for fastidious bacteria.

MacConkey Agar

A selective and differential media for Gram-negative bacteria, differentiating lactose fermenters from non-fermenters.

Sorbitol-MacConkey Agar (SMAC)

A variant of MacConkey agar that detects E. coli O157:H7, distinguishing it from lactose-fermenting strains.

Eosin Methylene Blue Agar (EMB)

A selective and differential media that differentiates lactose fermenters from non-fermenters based on colony color.

Mannitol Salt Agar (MSA)

A red selective and differential media for Gram-positive bacteria, differentiating pathogenic Staphylococcus species based on mannitol fermentation.