MT 6320: Gram-Negative Cocci - Neisseria & Moraxella catarrhalis

Comprehensive fill-in-the-blank practice flashcards covering the characteristics, virulence factors, clinical pathologies, selective media, and diagnostic differentiation of Neisseria and Moraxella catarrhalis.

All members of the genus Neisseria are Gram-negative diplococci with a coffee bean or kidney-like shape, except for N. weaveri, N. bacilliformis, N. animaloris, N. zoodegmatis, and __________.

N. elongata

All Neisseria species are catalase-positive except for N. elongata and __________.

N. bacilliformis

The optimal incubation temperature range for the growth of Neisseria species is __________.

32\text{--}36^\rig^\text{C}

Neisseria species possess an endotoxin composed of __________ rather than lipopolysaccharide.

Lipooligosaccharide

Among the members of the genus Neisseria, __________ is noted as not being a part of the human normal microbiota.

N. animaloris

The outer membrane protein of Neisseria that functions as a porin channel and includes PorA and PorB is __________.

Protein I

In pathogenic Neisseria, the cell membrane protein responsible for mediating adhesion to phagocytic and epithelial cells is Protein II, also designated as __________.

Opa

The outer membrane protein in Neisseria that specifically blocks IgG action is Protein III, also known as __________.

Rmp

Gonorrhea is defined as an acute pyogenic infection of __________ and transitional epithelia.

non ciliated columnar

A serious gonococcal complication in females characterized by perihepatitis and liver adhesions is __________.

Fitz Hugh Curtis Syndrome

Among the colony types of Neisseria gonorrhoeae, the virulent and pathogenic forms that possess pili are __________.

T1 and T2

The recommended class of antibiotics for the treatment of Neisseria gonorrhoeae infections is __________.

cephalosporins

Neisseria meningitidis possesses an antiphagocytic polysaccharide capsule that is classified into __________ serogroups.

$$12$$

A fulminant manifestation of meningococcemia marked by adrenal gland hemorrhage and DIC is known as __________.

Waterhouse Friderischen Syndrome

Among the serogroups of Neisseria meningitidis, serogroup __________ is predominantly associated with disease in underdeveloped countries.

A

For the laboratory diagnosis of gonorrhea in women, the specimen of choice is __________ discharge.

endocervical

When directly plating Neisseria gonorrhoeae onto a JEMBEC plate, the specimen is inoculated in a characteristic __________ pattern.

Z

Modified Thayer-Martin (MTM) agar contains vancomycin, colistin, nystatin, and __________ to inhibit the swarming of Proteus.

trimethoprim

Martin Lewis agar includes the antifungal agent __________ instead of nystatin.

anisomicin

New York City (NYC) Agar contains vancomycin, colistin, trimethoprim, and the antifungal agent __________.

amphotericin B

The oxidase test detects cytochrome C using the reagent 1% __________ dihydrochloride.

dimethyl-p-phenylenediamine

The Superoxol test, which produces an immediate and vigorous 4+ bubbling reaction in Neisseria gonorrhoeae, utilizes a concentration of __________ hydrogen peroxide.

$$30\%$$

Definitive diagnosis via carbohydrate utilization testing relies on Cystine Trypticase Soy Agar base with a __________ indicator.

phenol red

In carbohydrate utilization tests using Cystine Trypticase Soy Agar base, Neisseria gonorrhoeae oxidizes only __________.

glucose

In carbohydrate utilization testing, Neisseria meningitidis produces acid from glucose and __________.

maltose

The commensal organism Neisseria lactamica is differentiated from pathogenic Neisseria by its ability to utilize __________.

lactose

The immunological assay Phadebact identifies gonococcal antigens using monoclonal antibodies through the principle of __________.

coagglutination

To prepare cerebrospinal fluid for direct Gram staining of Neisseria meningitidis, $1\,\text{ml}$ of CSF is centrifuged at __________ for $10\,\text{mins}$.

$$1000\,g$$

Of the classic Neisseria pathogens, only __________ is capable of growing on both sheep blood agar and chocolate agar.

Neisseria meningitidis

The tetravalent meningococcal vaccine confers protection against serogroups A, C, Y, and __________.

W135

Moraxella catarrhalis colonies can be pushed intact across the surface of an agar plate, exhibiting a friable __________ consistency.

Hockeypuck

Unlike Neisseria species, Moraxella catarrhalis is asaccharolytic and gives a positive reaction for butyrate esterase and __________.

DNAse

Neisseria cinerea can be mistakenly identified as Neisseria gonorrhoeae, but unlike N. gonorrhoeae, it is capable of growing on __________.

BAP

The commensal species Neisseria sicca characteristically forms dry, wrinkled, __________-like colonies.

breadcrumb

The commensal organism __________ produces large quantities of extracellular polysaccharide when cultivated on media containing $1\%$ or $5\%$ sucrose.

N. polysaccharea