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Forensic science
Application of science to questions of law.
CSI Effect
Media portrayals can shape public and juror expectations about forensic evidence.
Forensic science vs. criminology
Forensic science applies scientific methods to legal questions; criminology studies crime and criminal behavior.
Reproducibility
Repeated scientific analyses under appropriate conditions should yield comparable results.
Falsifiability
A scientific claim can be tested in a way that could potentially show it is wrong.
Peer review
Other qualified scientists evaluate methods, data, and conclusions.
Contextual bias
Case information not needed for testing may influence an examiner’s interpretation.
Sequential unmasking
Providing information to analysts in a controlled order to reduce bias.
Blind sample
A test sample whose identity or expected result is unknown to the analyst.
Positive control
A sample expected to produce a positive result.
Negative control
A sample expected not to contain the target substance or characteristic.
SOP
Standard Operating Procedure; written instructions for performing laboratory work.
Accreditation
Formal recognition that an organization meets specified standards.
Certification
Recognition that an individual meets specified professional requirements.
2009 NAS report
Highlighted the need for stronger scientific foundations, validation, standards, and consistency in forensic science.
Four federal forensic laboratories
FBI, DEA, ATF, and U.S. Postal Inspection Service.
Preponderance of evidence
Civil standard meaning more likely than not.
Beyond reasonable doubt
Higher burden of proof required for criminal conviction.
Trier of fact
Judge or jury responsible for deciding factual issues.
Ethics
Objectivity, honest reporting, proper procedures, and acknowledging scientific limitations.
Locard’s Exchange Principle
Contact creates opportunities for transfer of physical material.
Direct evidence
Evidence that directly establishes a fact if believed, without requiring an inference.
Circumstantial evidence
Evidence requiring an inference to connect it to a fact.
Class characteristics
Characteristics shared by a group of items or sources.
Individual characteristics
Characteristics that may strongly associate evidence with a particular source.
Inculpatory evidence
Evidence tending to support involvement or guilt.
Exculpatory evidence
Evidence tending to support innocence or exclude involvement.
Inclusion/association
Findings consistent with a proposed source; not always unique identification.
Exclusion
Meaningful differences rule out a proposed common source.
Inconclusive
Findings do not reliably support inclusion or exclusion.
Admissibility
Whether evidence may be considered in court.
Frye standard
Emphasizes general acceptance of a scientific method in the relevant field.
Daubert standard
Considers testability, peer review, error, standards, validation, and acceptance.
Rule 702
Federal rule governing expert testimony.
Judge as gatekeeper
Judge evaluates whether proposed expert evidence meets admissibility requirements.
Evidence does not prove
An association alone does not establish when transfer occurred or that a crime was committed.
Crime scene
Any location associated with an event under investigation.
Primary scene
Main location where the event of interest occurred.
Secondary scene
Additional location associated with the investigation.
Macro scene
Larger overall scene or area being investigated.
Micro scene
Smaller area or feature within a larger scene.
First responder priority
Protect life and safety before evidence preservation.
Entry log
Record of who entered a scene, when, and why.
Elimination sample
Known sample from someone legitimately present to help distinguish innocent traces.
Preliminary survey
Initial walk-through to identify hazards, evidence, boundaries, and processing needs.
Documentation methods
Notes, photos, video, sketches, measurements, mapping, and 3D scanning.
Photography sequence
Overall views, intermediate views, then close-ups.
Rough sketch
Sketch made at the scene showing layout, measurements, and evidence locations.
Final sketch
Cleaned-up representation based on original scene measurements.
LiDAR
Uses laser measurements to produce detailed 3D representations.
Search patterns
Systematic methods such as line, grid, spiral, zone, and wheel/ray.
Chain of custody
Documented record of evidence possession and transfers.
Why chain of custody matters
Helps establish evidence integrity and accountability.
Packaging separate items
Reduces cross-contamination.
Wet biological evidence
Should be handled to allow proper drying; avoid sealing wet material in airtight plastic.
Paper packaging
Commonly used for properly dried biological evidence because moisture can escape.
Control sample
Comparison material used to interpret an evidence sample.
Collection order
Locate, document, collect, package, secure, and record transfers.
Trace evidence
Small transferred materials such as fibers, glass, soil, paint, and residues.
Primary transfer
Direct movement of material from its original source to another surface.
Secondary transfer
Movement of material through an intermediate person or object.
Questioned sample (Q)
Sample recovered during investigation whose source is uncertain.
Known sample (K)
Reference sample from a documented source.
Stereo microscope
Provides a 3D-like view useful for preliminary examination and particle recovery.
Compound microscope
Uses transmitted light and higher magnification for suitable specimens.
Total magnification
Objective magnification multiplied by eyepiece magnification.
PLM
Polarized-light microscopy; useful for fibers, minerals, and optical properties.
Isotropic
Optical properties do not change with direction.
Anisotropic
Optical behavior changes with direction.
Birefringence
Difference in refractive indices associated with anisotropic materials.
Comparison microscope
Allows simultaneous side-by-side examination of two specimens.
MSP
Microspectrophotometry; objectively measures color characteristics.
Raman spectroscopy
Uses light scattering to obtain molecular information.
SEM/EDS
SEM images small features; EDS measures elemental composition.
Radial glass cracks
Cracks extending outward from an impact point.
Concentric glass cracks
Cracks forming around an impact point.
Refractive index
Optical property describing how light travels through a material.
Becke line
Bright halo used in refractive-index comparison.
Fiber comparisons
May examine color, morphology, cross-section, optical behavior, and chemistry.
Paint comparisons
May examine color, layer sequence, thickness, pigments, and composition.
Soil comparisons
May examine minerals, color, pH, particles, pollen, and optical properties.
Gunshot residue (GSR)
Inorganic particles associated with firearm discharge and ammunition primer.
GSR analysis
SEM/EDS evaluates particle shape and elemental composition.
Why “match” can be misleading
Shared class characteristics do not necessarily prove a unique common source.
Medicolegal death investigation
Investigation of questionable, sudden, suspicious, or unexplained deaths.
MDI
Medicolegal death investigator who examines circumstances, body, and scene information.
Coroner
Death-investigation official whose qualifications vary by jurisdiction.
Medical examiner
Physician-based death investigation official/system.
Forensic pathologist
Physician specializing in medical investigation of death and autopsy interpretation.
Cause of death
Disease or injury that begins the lethal chain of events.
Mechanism of death
Physiological or biochemical failure produced by the cause.
Manner of death
Natural, accident, suicide, homicide, or undetermined.
Homicide classification
Death caused by another person; not itself a legal finding of guilt.
PMI
Postmortem interval; estimated time elapsed since death.
Rigor mortis
Postmortem stiffening of muscles.
Rigor timing
Generally begins around 4 hours and may disappear around 24–36 hours; highly variable.
Livor mortis
Blood settling after death, causing discoloration.
Fixed lividity
Lividity that no longer blanches under pressure; often around 12 hours.
Algor mortis
Cooling of the body after death.
Algor rough estimate
About 1.5°C per hour for first 8 hours under specified conditions; not a universal rate.