card 3 SANDWICH ELISA

11. What is attached to the plate in sandwich ELISA?

A. Detection antibody

B. Capture antibody

C. DNA probe

D. Secondary antibody only

Correct Answer: B. Capture antibody

🧠 Explanation: Sandwich ELISA wuxuu ku bilaabmaa capture antibody oo plate-ka ku coated ah.

12. In sandwich ELISA, the antigen is:

A. Bound between two antibodies

B. Bound between two DNA strands

C. Destroyed by the enzyme

D. Bound only to the substrate

Correct Answer: A. Bound between two antibodies

🧠 Explanation: Capture antibody ayaa antigen-ka qabata, kadib detecting antibody ayaa ku xirma—sidaas ayuu “sandwich” u noqdaa.

13. Why is sandwich ELISA highly specific?

A. It uses two antibodies

B. It uses two DNA probes

C. It uses no antibodies

D. It only detects RNA

Correct Answer: A. It uses two antibodies

🧠 Explanation: Two antibodies ayaa target antigen-ka aqoonsada, sidaas darteed specificity-gu waa sarreeyaa.

14. Which type of sample is particularly suitable for sandwich ELISA?

A. Complex samples

B. Pure DNA only

C. RNA only

D. Purified ribosomes only

Correct Answer: A. Complex samples

🧠 Explanation: B&B waxay sheegaysaa sandwich ELISA inuu ku fiican yahay complex samples, antigen-kuna uma baahna purification.