protocol 1&2- pipette background
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29 Terms
what pipette
pasture pipette
pasteur pipette
made of glass and have a long, tapered end that allows access to small spaces
what is pastur pipette NOT used for
for the transfer of accurate volumes
not graduated
what pipette is this
serological pipette
serological pipette
are glass or plastic that are graduated for specific volumes
how is liquid drawn from serological pipette
suction device
what pipette is this
transfer
transfer pipette
have volume markers to estimate volume aspirated and dispensed
when should you use a transfer pipette
if accuracy is not critical
what pipette is this
piston-driven air displacement pipettes
piston-driven air displacement pipettes
used for dispensing accurate volumes less than 1ml but can have more than 1ml
piston-driven air displacement pipettes dial
is set for a specific volumes and must use appropriately sized tips
piston-driven air displacement pipettes are what
extremely accurate
what pipette is this
volumetric pipette
volumetric pipette
designed to accurately transfer a specified volume
how does liquid work in volumetric pipette
it is aspirated with a suction device and dispensed by the force of gravity
a volumetric pipette different designs
to deliver or to contain a specified volume
volumetric pipette (to deliver) what NOT to do
force out the last bit of liquid contained in the pipette
what pipette is this
capillary pipette
capillary pipette
like volumetric pipettes but are intended for very small amounts
how is liquid moved in capillary pipette
liquid is aspirated by capillary action and dispensed by the force of gravity
how is capillary pipette set
may be graduated or intended for a set volume
how is liquid moved in pasteur pipette
a suction bulb
serial dilution
substance in solution being diluted in a stepwise manner, using a constant dilution factor at each step
when would serial dilution be used
experiments that require that use of concentrations curves, trendlines, and for plating cells
what plate do we use for serial dilution
96 well
plate reader does what
collect the absorbance value of each well of the plate
whats the range used to detect red indicator
562 nm
R2 value
used to determine how well the linear line fits the data