SCV 213: Lab White Blood Cells

Normal White Blood Cell Count

Feline: 3.8-19 10³ uL

Canine: 5.9-16.6 10³ uL

Total White Blood Cell formula

Average x 50/ 1000 = __________ 10³ uL

Relative count of WBC differential

Ex: 80 neutrophils = 80% for the relative count

Absolute count of WBC differential

Relative % / 100 x WBC count (10³ uL) = ________ x 10³ uL

Stains for WBC

Known as Diff Quik in 4 components:

1.) Fixative (aqua blue) - 95% methanol, fixes cells to slide

2.) Eosin (reddish orange) - Acidic pH, attracted to alkaline cell cytoplasm. Stains hemoglobin and eosinophilic granules pink to reddish

3.) Methylene Blue or New Methylene Blue (purple blue) - Alkaline pH, attracted to acidic (DNA, RNA). Stains nuclei and basophilic granules blue to purple

4.) Rinse - Distilled water to rinse stain off slides

Semi-quantification scale

Scale of 1+, 2+, 3+, 4+
Indicates the relative percentage of cells with changes
◦ 1+ = 5% to 10% of cells
◦ 2+ = 10% to 25% of cells
◦ 3+ = approximately 50% of cells
◦ 4+ = more than 75% of cells