Biochem Lect 13.5 + 14.5 NOT FINISH

Krebs cycle, Citric Acid Cycle, TCA

Where does Krebs take place in the cell?

Mitochondrial matrix

Metabolons???

Functional units of enzymes

What is the difference between acetyl & acyl?

Acyl has a general formula (R-CXO), while acetyl has (H3C-CXO)

What is pyruvate dehydrogenase complex (PDC) comprised of?

E₁ + E₂ + E₃

E₁ name, cofactors & f(x)

Pyruvate dehydrogenase, w/ TPP coenzyme

Decarboxylates pyruvate and remaining molecule (hydroxyethyl) is held by TPP

E₂ name, cofactors & f(x)

Dihydrolipoyl transacetylase, w/ Lipoic acid/CoA-SH.

Lipoyllysine receives hydroxyethyl from TPP, reducing S, and transfers the now acetyl group to CoA

E₃ name, cofactors & f(x)

Dihydrolipoyl dehydrogenase, w/ FAD & NAD⁺

FAD oxidizes the lipoyllysine to renew E₂, and the now FADH₂ then gets oxidized by free NAD⁺ to renew E₃

State all the byproducts of Krebs in order

1.) Makes CoA-SH
2.) None
3.) Consumes NAD(P)⁺ and creates NAD(P)H + H⁺ + CO₂
4.)