Cell and Cancer Bio - Exam 2

Which of the following are RTKs?

• RDGFR

• Her2 (ErbB2)

• Smoothened

• Frizzled

• IGF-1R

• Patched

• Type II TGF-beta receptors

RDGFR, Her2 (ErbB2), IGF-1R

Which of the following are oncogenes?

• p53

• Fos

• Ras

• Akt

• Tsc2

Fos, Ras, Akt

Which of the following are transcription factors?

• myc

• Gli

• CREB

• Ras

• Erk

• Tsc2

• SmMAD4

myc, Gli, CREB, SMAD4

Which of the following are Tumor Suppressor Genes?

• Rheb

• mTOR

• TSC2

• AMPK

• Ras

• Patched

• ERK

• TSC1

• PTEN

• Smoothened

• PI3K

TSC2, Patched, TSC1, PTEN

Which of the following are related to oncogenes?

• mutations in both alleles are needed

• promoting cell growth is their normal function

• large deletions

• loss of heterozygosity

• activating mutations

• gene amplification

• viral integration

promoting cell growth is their normal function, activating mutations, gene amplification, viral integration

Which of the following are related to tumor suppressor genes?

• mutations in both alleles are needed

• viral integration

• activating mutations

• normal function is cell growth inhibition

• out-of-frame insertions / deletions (indels)

• loss of heterozygosity

mutations in both alleles are needed, normal function is cell growth inhibition, out-of-frame insertions / deletions (indels), loss of heterozygosity

Which one is a loss of heterozygosity?

• the activation of proto-oncogenes due to gene fusion

• the loss of a proto-oncogene due to gene fusion

• the loss of heterozygous polymorphic markers in the vicinity of a tumor suppressor gene due to large chromosomal deletions

• the loss of tumor suppressor genes due to large chromosomal deletions

the loss of heterozygous polymorphic markers in the vicinity of a tumor suppressor gene due to large chromosomal deletions

Which of the following are GTPases?

• Gli

• SMAD4

• Tsc2

• Rho

• CREB

• Rag

• Rheb

• Ras

Rho, Rag, Rheb, Ras

Which of these kinases are directly phosphorylating ribosomal protein S6 (RPS6)?

• AKT/PKB

• PI3K

• AMPK

• Erk1/2

• RSK1 (p90S6K)

• mTORC1

• mTORC2

• p70S6K

RSK1 (p90S6K), p70S6K

Which of the following kinases directly phosphorylate Akt/PKB?

• ERK1/2

• PDK1

• PI3K

• mTORC1

• p70S6K

• RTKs

• AMPK

• mTORC2

PDK1, mTORC2

Which of the following are substrates of Akt/PKB?

• Bcl-2

• mTORC2

• PTEN

• PI3K

• PDK1

• AMPK

• mTORC1

• p70S6K

• Rheb

• BcI-xL

• TSC2

• Bad

TSC2, Bad

Which of the following are directly sensitive to rapamycin?

• mTORC2

• AKT

• mTORC1

• MTOR (kinase)

• AMPK

• PI3K

mTORC1

Which component of protein of mTORC2 is a substrate of p70S6K

• mSIN1

• Protor

• mLST8

• Depor

• mTOR (kinase)

• Rictor

Rictor

Which of the following creates phosphatidylinositol 4,5-biphosphate (also known as PIP2)?

• PI kinase

• PI3K

• PLC-beta

• PIP kinase

• PTEN

• Akt/PKB

PIP kinase, PTEN

Which one of the following kinases would be most sensitive to metformin?

• mTORC2

• Akt/PKB

• PI3K

• p70S6K

• AMPK

• mTORC1

AMPK

How many members are in the TGF-beta family of ligands?

• 3

• more than 20

• between 10 and 20

• less than 10

• about 30

about 30

TGF-beta family ligand dimers bind first to…

• SMADs

• Type I receptors

• Type II receptors

Type II receptors

Which one is true for TGF-beta signaling?

• TGF-beta type I and type II receptors have an intracellular tyrosine kinase domain

• TGF-beta type I and type II receptors have an intracellular serine/threonine kinase domain

• TGF-beta type I receptors have an intracellular serine/threonine kinase domain, whereas type II receptors have an intracellular tyrosine kinase domain

• TGF-beta type I receptors have an intracellular tyrosine kinase domain, whereas type II receptors have an intracellular serine/threonine kinase domain

• TGF-beta type I and type II receptors are RTKs

TGF-beta type I and type II receptors have an intracellular serine/threonine kinase domain

Which of the following are true for Sonic Hedgehog (SHH) signaling?

• Smoothened inhibits Patched

• Patched is a GPCR

• Gli in invertebrates is equivalent to Ci in vertebrates

• Full-length Gli (Ci) is a transcriptional factor

• The Gli/Fused/Suppressor of Fused protein complex is sequestered to the microtubules by Coastal-2 (Cos2)

• The ligand (SHH) binds to Patched

Full-length Gli (Ci) is a transcriptional factor, The Gli/Fused/Suppressor of Fused protein complex is sequestered to the microtubules by Coastal-2 (Cos2), the ligand (SHH) binds to Patched