FLUOROMETRY/CHEMILUMINESCENCE

fluorometry

• analytical technique that measures fluorescence to detect and quantify substances in a sample

energy emission

• occurs when certain compounds absorb electromagnetic radiation, becomes excited gives off light

photometric

• measurement of light emitted by a substance that has been previously excited by a source of UV light

fluorometer

• measure the concentrations of solution that contain fluorescing molecules

• device that measures the fluorescence, which is the light emitted by a sample after absorbing excitation light

chemiluminiscence

• no excitation radiation is required and no monochromator are needed because this procedure arises from one specie

• this emits light from the electronically excited state of a sample produced by a chemical reaction, rather than from external light source

luminol

• a chemical that glows with a blue light when it reacts with an oxidizing agents, a process called chemiluminiscence

• reacts with the heme in hemoglobin (blood at a crime scene)