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Kahoot & study guide material!
What are the 2 methods of obtaining a total WBC count?
Automatic and manual
3 types of hematology analyzers
Independence analyzers, laser flow cytometry, quantitative buffy coat analysis
What is the name of the piece of equipment that is designed for manually counting skills?
Neubauer hemocytometer
What is the medical term for increased WBC count?
Leukocytosis
What is the medical term for decreased WBC count?
Leukopenia/leukocytopenia
A stress leukogram is caused by _______ and results in a _______.
Illness, lymphopenia
A physiological leukocytosis is caused by _______ and results in a ________.
Anxiety, lymphocytosis
Normal range for total canine WBC counts?
6,000-15,000/uL
Normal range for feline total WBC count?
5,500-19,500/uL
2 causes of leukocytosis
Pathogens, leukemia
2 causes of leukopenia
Viral infections, bone marrow disease
What objective do we use during the WBC differential?
100x, oil immersion
How many WBC’s do we count?
100
Where on the slide do we look for WBC?
Monolayer
What is the difference between the relative and absolute count?
Relative is a percentage, less accurate, and not used for diagnostics. Absolute is a whole number, more accurate, and is used for diagnostics.
Which is more accurate?
Absolute count
What is the formula for obtaining the absolute count?
Relative count x Total WBC
What are the units we use to measure the absolute count?
/uL
Canine relative for neutrophils?
60-70%
Feline relative for neutrophils?
35-75%
Canine absolute for neutrophils?
3,000-11,300/uL
Feline absolute for neutrophils?
2,500-12,500/uL
Increase of neutrophils:
Neutrophilia
Decrease in neutrophils:
Neutropenia
Canine relative for lymphocytes:
12-30%
Feline relative for lymphocytes:
20-55%
Canine absolute for lymphocytes:
1,000-4,800/uL
Feline absolute for lymphocytes:
1,500-7,000/uL
What animals are lymphocytes most abundant in?
Ruminants (Cattle, rabbits, some rodents)
Increase in lymphocytes:
Lymphocytosis
Decrease in lymphocytes:
Lymphopenia
True or false: Animals under 6 months of age can have increased lymphocyte counts
True
Canine relative for monocytes:
3-9%
Feline relative for monocytes:
1-4%
Canine absolute for monocytes:
200-1,300/uL
Feline absolute for monocytes:
0-900/uL
Monocytes become _______ once migrating to tissues
Macrophages
Monocytes are indicative of ______ infection or inflammation
Chronic
Canine relative for eosinphils:
2-10%
Feline relatives for eosinophils:
2-10%
Canine absolute for eosinophils:
100-750/uL
Feline absolute for eosinophils:
0-800/uL
When do we see eosinophilia most commonly?
Allergies and parasitic infections
Canine relatives for basophils:
0-3%
Feline relative for basophils:
0-3%
Canine absolute for basophils:
0-30/uL
Feline absolute for basophils:
0-30/uL
When might we see basophils in peripheral blood?
Severe allergies and parasites
What cell is often seen with basophils?
Eosinophils