BIO 1010 Unit 2 Lab exam

What does Benedicts test for

glucose

what deos iodine test for

starch

what does sudan IV test for

lipids

what does biuret test for

proteins

benedict pos/neg colors

pos: green-red

neg: blue

iodine pos/neg colors

pos: black

neg: brown

sudan IV pos/neg colors

pos: dark pink-red

neg: light pink

biuret pos/neg colors

pos: purple

neg: blue

benedicts test steps

1. fill test tube with 1cm solution and another test tube with 1cm dH₂O

2. put a dropper of benedicts in both tubes (shake)

3. boil tubes for 2-5 minutes

4. take test tubes out and observe color

iodine test steps

1. fill test tube with 1cm solution and another test tube with 1cm dH₂O

2. put a dropper of iodine in both tubes (shake)

3. observe color

biuret test steps

1. fill test tube with 1cm solution and another test tube with 1cm dH₂O

2. put a dropper of biuret in both tubes (shake)

3. observe color

sudan IV test steps

1. on filter paper draw and label two circles solution and dH₂O

2. put a dropper of solution and dH₂O in respective circles

3. use dryer to fully incorperate drops into filter paper

4. saturate filter paper in a petri dish of sudan IV solution for 3 minutes

5. get a petri dish of dH₂O and place filtered paper in it for a minute

6. observe color change

lettuce lab results

sugar: +

starch: -

protein: -

lipid: -

hamburger lab results

sugar: -

starch: -

protein: +

lipid: +

tuna lab results

sugar: -

starch: -

protein: +

lipid: -

milk lab results

sugar: +

starch: -

protein: +

lipid: +

white beans lab results

sugar: +

starch: +

protein: +

lipid: +

almond butter lab results

sugar: +

starch: -

protein: +

lipid: +

g to carb/kcal formula

carbs/g x 4=kcal

protein/g x 4=kcal

fat/g x 9=kcal

percentage of kcal consumed formula

(total kcal in category/total kcal consumed)100= %

recommended % of intake for carbs, protein, and fat

carbs: 55-60%

protein: 10-15%

fat: no more than 30%