STREP

LAP TEST (Leucine Amino Peptidase Test)

to detect gram positive cocci that are catalase negative

Leucine Beta naphthylamide

filter paper disk impregnated with substrate ____ _________ _______

D-dimethyl amino cinnamaldehyde

Color developer: __________

red

LAP test (+) result ______

Streptococci

Gram positive cocci in chains, spherical to ovoid

Streptococci

Catalase (-), Non-motile; Capnophilic

5% SBA

Medium of Choice: __________ for strep

PEA agar

Selective Medium: ________ for strep

Enterococcus, Streptococcus, Pediococcus

LAP TEST (+)

Aerococcus & Leuconostoc

LAP TEST (-)

hemolysis

Starting point in the identification of Streptococci is to determine ____________ pattern

5% SBA; PEA, CNA

Routine media for Isolation (medium of choice) ________________________, other media _________________________

S. pyogenes & S. agalactiae

Grows only at 37 degC

pyogeic strep

Grows only at 37 degC at called

S. mutans, S. mitis

Grows at 37 & 45 degC

Viridans strep

Grows at 37 & 45 degC is called

S. lactis

Grows at 10 & 37 deg

Lactic strep

Grows at 10 & 37 deg is called

E. faecalis, E. faecium

Grows at 10, 37 & 45 degC

enterococci

Grows at 10, 37 & 45 degC are called

alpha

Can cause incomplete/partial hemolysis on BAP

alpha

Can cause greening of agar

alpha

Streptococcus pneumoniae, Viridans Streptococci – S. mitis and S. mutans

beta

Can cause complete hemolysis on BAP

beta

Can produce clear zones around the colonies

beta

S. pyogenes & S. agalactiae hemolysis

gamma

Do not cause hemolysis on BAP

Group D Non-Enterococci / Enterococci

LANCEFIELD

LANCEFIELD

LANCEFIELD

S. pyogenes

LANCEFIELD group A

s. agalactiae

LANCEFIELD group B

S. dysgalactiae, S. equi

LANCEFIELD group C, G

S. bovis, Enterococci

LANCEFIELD group D

alpha prime (wide)

colonies are surrounded by a very small zone of no hemolysis and then a wider zone of β-hemolysis. This reaction may be mistaken for β-hemolysis at first glance.

double/target

inner beta, outer alpha

C perfringens

double target is seen on ______

viridans strep

Organism possible for alpha prime zone

10% BAP

BILE SOLUBILITY TEST Media: ____________

sodium desoxycholate

BILE SOLUBILITY TEST To be added: __________

lysis

BILE SOLUBILITY TEST (+) result in ____ of colonies

intact

BILE SOLUBILITY TEST (-) result ______ colonies

clearing

BILE SOLUBILITY TEST (+) result if on broth _____ of media

turbid/partial

BILE SOLUBILITY TEST (-) result if on broth _____ clearing

Positive

BILE SOLUBILITY TEST S penumoniae

negative

BILE SOLUBILITY TEST VIRIDANS

positive

CAPSULAR SWELLING TEST NEUFELD QUELLUNG S. penumoniae

negative

CAPSULAR SWELLING TEST NEUFELD QUELLUNG VIRIDANS

TAXO P

Optochin is also known as ______

Ethylhydrocuprein hydrochloride

Chemical name of Optochin is –

Mueller hinton + blood agar

OPTOCHIN DISK TEST media

TAXO P

after inoculation for OPTOCHIN DISK TEST we apply _____

inhibition

clearing in OPTOCHIN DISK TEST is zone of _____

14mm

Considered susceptible in OPTOCHIN DISK TEST base on antibiotic width 6mm

18mm

Considered susceptible in OPTOCHIN DISK TEST base on antibiotic width 10mm

Sensitive

OPTOCHIN DISK TEST s. pneumoniae

resistant

OPTOCHIN DISK TEST VIRIDANS

Streptococcus pneumoniae

Normal flora of nasopharynx and oropharynx (URT)

adult bacterial meningitis

Streptococcus pneumoniae is regarded as # 1 cause of _________________________________, can cause LOBAR PNEUMONIA and OTITIS MEDIA

polysaccharide capsule

Streptococcus pneumoniae Major Virulence factor ______________________

LANCET

Streptococcus pneumoniae microscopically appears as ______ or BULLET SHAPED DIPLOCOCCI

alpha

Colony Morphology of Streptococcus pneumoniae

dome shape

Streptococcus pneumoniae: After 24 hrs of incubation (young colonies) colonies may appear ___________________

crater-like

Streptococcus pneumoniae: After 48 hrs of incubation (aged colonies) growth may appear _____________________________, like a coin with a raised rim, Nail head appearing colonies or colonies with checker appearance.

VIRIDANS Streptococci

normal microbiota of the upper respiratory tract, the female genital tract, and the gastrointestinal tract

Lancefield

VIRIDANS Streptococci is not classified under ___________

glucans and dextrans

VIRIDANS produces ______________ & adhesins – promotes attachment on tooth surfaces

throat

VIRIDANS Regarded as the major ______________ flora

Sub acute bacterial endocarditis

VIRIDANS #1 cause of ____________________ And contributory to dental carries

S. mutans

_____________group is the most commonly isolated among the Viridans streptococci.

S. mutans

_____________primary contributor to dental caries. It is also, the most common member of the mutans group associated with bacteremia