Additional hematologic tests

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Last updated 11:43 PM on 9/26/26
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7 Terms

1
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Reticulocytes

  • is a immature RBC with no nucleus

  • stain is done with new methane blue

  • horses have no reticulocytes

  • absolute reticulocyte is the most accurate assessment of erythropoiesis


2
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feline reticulocytes

  • aggregate and punctate

  • aggregate is the only one we count


3
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reticulocyte count

  • expression of the percentage of RBCs that are reticulocytes

  • percentage per 1000 RBCs

  • not done on horses since they do not release reticulocytes

  • higher percentage - hemolytic anemia

  • lower percentage- hemorrhagic anemia


4
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regenerative anemia

  • functional bone marrow actively responding to RBC loss or destruction

  • evidenced by elevated reticulocyte count, polychromasia (multiple colors), anisocytosis, macrocytosis (high MCV), hypochromasia (low MCHC)


5
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Bone marrow evaluation

  • indications > unexplained persistent pancytopenia (Decrease in all types of blood cells), non regenerative anemia, atypical/immature cells in circulation

  • collection sites > proximal humerus


6
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blood smear time

  • must be done within an hour of collection and an EDTA tube should be used


7
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corrected reticulocyte count equation

  • multiply observed retics by the observed PCV and then divide by normal PCV


normal PCV: dog- 45%; cat- 35%