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Light Microscope (LM)
Used to visualize cells
Electron Microscopes (EMs)
Used to study subcellular structures
Scanning electron microscopes (SEMs)
Focuses a beam of electrons onto the surface of a specimen, providing images that look 3-D
Transmission electron microscopes (TEMs)
Focuses a beam of electrons through a specimen, used to study the INTERNAL structure of cells
Cell fractionation
Takes cells apart and separates the major organelles from one another, helps determine the function of organelles
Basic cell types
Eukaryotic (plants, animals, fungi) & prokaryotic (bacteria) cells
Basic features of all cells
Plasma membrane
Semifluid substance called cytosol
Chromosomes (carry genes)
Ribosomes (make proteins)
Eukaryotic cells have…
DNA bound by nuclear envelope
Membrane-bound organelles
Cytoplasm
Mitochondria
Prokaryotic cells have…
No mitochondria
Cell suface area to volume ratio
As a cell increases in size, its volume grows proportionately more than its surface area, limits cell size
Nucleus
Contains most of the cell’s genes and is usually the most conspicuous organelle
Nuclear envelope
Encloses the nucleus, separating it from the cytoplasm
Chromosomes
Single units of DNA molecule
Chromatin
DNA and protein of chromosomes bind together
Nucleolus
Located within the nucleus and is the site of ribosomal RNA (rRNA) synthesis
Ribosomes
Complexes made of ribosomal RNA and protein, carry out protein synthesis
Endomembrane system
Regulates protein traffic and performs metabolic functions in the cell, consists of
Nuclear envelope
Endoplasmic reticulum
Golgi apparatus
Lysosomes
Vacuoles
Plasma membrane
Smooth ER
Synthesizes lipids
Metabolizes carbohydrates
Detoxifies drugs and poisons
Stores calcium ions
Rough ER
Has bound ribosomes, which secrete glycoproteins (proteins covalently bonded to carbohydrates)
Distributes transport vesicles, secretory proteins surrounded by membranes