Q'S FOR EXERCISE 14 CATALASE AND OXIDASE
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16 Terms
what is the substrate for catalase test
hydrogen peroxide
what enzyme catalyzes hydrogen peroxide
catalase
what process is happening during catalase test
reducing toxic hydrogen peroxide into safe byproducts
what products are produced in catalase test
water and oxygen gas
how do you visualize catalase test
observing bubbling on the glass slide
what reagent must be added to visualize catalase test
hydrogen peroxide
what does positive catalase test look like
bubbles form immediately
what does negative catalase test look like
no bubbles form
what is the substrate for oxidase test
reduced electron carriers in electron transport chain
what enzyme catalyzes in
what is the process happening during oxidase test
fully reducing oxygen into water at the end of the electron transport chain
what products are produced in oxidase test
water
how do you visualize oxidase test reaction
checking for a color change on a test strip
what reagent is added to visualize oxidase test
no reagent, dry slide is impregnated with oxidase reagent
what does positive oxidase test look like
a dark purple to black color appearing within 20 seconds
what does a negative oxidase test look like
no color change within 20 seconds