Microbio Lab (Exam 2): Microscope + extra stuff

Selective media

inhibits growth of certain bacteria.

Differential media

visualizes metabolic differences.

Resolution

clarity/sharpness of an image.

Limit of resolution or resolving power

minimum distance between two objects to be distinguished.

Numerical aperture

light gathering ability of objective lens and condenser.

Condenser

collects and focuses light onto specimen.

Ocular lens

eye piece lens that contributes to magnification (usually 10x).

Iris diaphragm

controls the amount of light entering microscope.

Objective lens are parfocal, meaning:

focus is conserved/standardized between different lenses.

Scanning lens

4x

Low power lens

10x

High-dry lens

40x