Nikon LMS - Ti2 Inverted Microscope

Perfect Focus System (PFS)

Anti drift mechanism

Causes of focal drift

Thermal fluctuations

Sample movement

Vibrations

What kind of light does PFS use?

Infrared beam (855nm)

How much liquid in sample chamber do you need for PFS to work correctly for aqueous samples?

3mm

How does PFS measure focus?

Location of IR beam on line CCD (PFS detector unit)

Does PFS track sample or coverslip position?

Coverslip

How does PFS track sample position?

By using PFS offset lens, which tracks sample position relative to coverslip

NI-BB Box

Nikon Trigger Box - provides high precision synchronization and triggering between microscope and devices

TTL (Transistor-Transistor Logic)

For devices that only can be ON/OFF (binary values)

Analog

For devices that have different levels of voltage (continuously variable values)

What does BNC cable stand for?

Bayonet Neill-Concelman