Complementation

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Last updated 7:30 PM on 2/26/25
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10 Terms

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Allelic mutations
Mutations in the same gene.
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Non-allelic mutations
Mutations in different genes.
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Wild-type (WT) strain
A normal strain that can grow on minimal media (prototroph).
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Auxotrophic strain
A mutant strain that cannot grow on minimal media and requires additional nutrients.
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Complementation test
A genetic test to determine whether two mutations affect the same gene or different genes.
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Complementation group
A group of mutants that fail to complement each other, indicating they belong to the same gene.
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Steps of a Complementation Test
1. Cross two haploid mutant strains with identical phenotypes (both auxotrophic). 2. Grow the resulting diploid strain on minimal media.
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Interpreting Growth in a Complementation Test
Growth indicates non-allelic mutations (mutations are in different genes); No growth indicates allelic mutations (mutations are in the same gene).
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Complementation results interpretation: Growth
Complementation occurs, mutations are in different genes (non-allelic).
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Complementation results interpretation: No growth
No complementation, mutations are in the same gene (allelic).