Non-Fouling & Non-Thrombogenic Surfaces

for BIOE 3020- Exam 2

PEG: poly(ethylene glycol) mechanisms to reduce protein adsorption

osmotic repulsion: extreme hydrophilicity, can’t remove water even at -100 celcius

entropic repulsion: surface-bound water is held in an extended configuration and protein adsorption would require compacting, this would decrease entropy and is therefore unfavourable

Pegylation

covalently conjugate PEG to bioactive small molecule (protein, nanoparticle, cytokine)

heparin is found where

endothelial tissue

what type of macromolecule is heparin

polysaccharide

when is heparinization used

blood-contacting devices

zwitterionicity

positive and negative ion in one molecule = neutral charge

zwitterionicity and non-fouling

high water binding = less protein adsorption

activated partial thromboplastin time (APTT) test

measuring time for a blood clot to form in the intrinsic and common pathway

prothombin time (PT) test

measure time for blood clot to dorm in extrinsic and common pathway

thrombosis

pathological/chronic clotting

embolism

blood clot blocking a vein/artery

GPIIb/IIa complex

platelet integrin receptor that bind to surface during adsorption