Bio Lab Practice Questions

Soda is a base?

Micropipette

Less than 1 mL

Serological Pipette

1-10 mL

Graduated Cylinder

More than 10 mL

What’s the basic unit of mass for the metric system?

Kilograms

Molarity is expressed as:

mol/L

When using a serological pipette or graduated cylinder, which of the following is the correct way to read the measured volume?

At the bottom of the meniscus at eye level

A p-value of > 0.05 is:

Not statistically Significant

A p-value of <0.05 is:

Statistically significant

The error bars we created in the bar/column graph represent:

Standard Deviation

Which molecule was used to calculate the M (molarity) of diet soda?

Aspartame

What material should you use to clean the lenses on a microscope?

Kleenex

If the total magnification of your specimen is 100X and you are using a 10X eyepiece, what is the magnification of your objective?

10X

Which liquid will be used to make a wetmount of both specimens/samples (Elodea leaf and check cells)?

Water

Should you use the coarse adjustment (focus) knob when viewing specimens/samples with a higher objective lens (e. g. 100X)?

No

Which objective lens(es) in chapter 4 lab was/were used with oil?

100X

As you increase the power of your objective lens (e.g. from 10X to 40X), your working distance (between the stage and objective) _________?

Decreases

Which structure(s) should you be able to see in the Elodea leaf specimen?

Which of the following is a white blood cell (leukocyte) that may have been seen in your blood smear slide?

Neutrophils

The Leukemia blood sample had on average _____ white blood cells per field compared to a normal blood sample

More

To answer questions in science and establish causal relationships, competency in the following areas are required:

Deductive reasoning moves from:

General to specific

A scientific hypothesis need to be ____ and _____.

Testable and Falsifiable

In Biology, and most sciences, statistical analysis allows for a ___%  margin of error.

5%

A hypothesis and prediction _____ the same thing (however they are related).

Are not

A hypothesis is ___ when the result of an experiment is consistent with your prediction and hypothesis.

Supported

Inductive reasoning:

Specific to general

Induction often helps ________.

generate the initial hypothesis

Deduction is used to _______.

derive testable predictions from the initial hypothesis.

The hypothetico-deductive method establishes that scientific research is entirely deductive and bypasses inductive reasoning altogether.

False

A control is subjected to the same conditions as the experimental subject/group, except for the variable being tested.

True

In chapter 7, how many Petri dishes will you use for each group/treatment to obtain reliable results (not including the viability  control)?

3

In chapter 7, ______ of each plate will be used as an experimental side?

the uncovered side

All microbiological waste (e.g. E. coli) material gets discarded in the normal trash?

False

In Chapter 7, what was the UV lamp's wavelength in um (micrometers)?

0.302 um (micrometers)

In Chapter 7, % survival = (_______ / ______) X 100.

Experiment divided by control

Diffusion is defined as the process where molecules move from an area of:

High to low

Osmosis is the diffusion of a ____ across a selectively permeable membrane along a concentration gradient.

Solvent

Dialysis is the diffusion of a ______ across a selectively permeable membrane along a concentration gradient.

Solute

When the concentration of solute in the (extracellular) solution is less than the concentration of solute in the cells, then the cell is in a(n)  ____ solution.

Hypotonic (Hypo hovers)

Which of the following materials will you be using during Chapter 8 lab?

Agar plate, Elodea leaf, potato, & dialysis tubing

The IKI solution will turn blue/black in the presence of ____.

Starch

In Chapter 8 experiment 1, the rate of diffusion results can be illustrated using a ____. 

Line graph (XY scatter plot) 

Given the equation of a line (y = mx + b), which  component represents the slope of the line?

M

In Chapter 8 experiment 2, the color of the solution _______ of the dialysis tubing should be dark blue/black after 30 minutes.

Inside

In Chapter 8 experiment 3, the 15% salt solution was considered a ____ solution (when compared to the Elodea leaf saline concentration).

Hypertonic

In Chapter 8 experiment 4, the % change in mass results can be illustrated using a ____.

Column (bar) graph

Molarity of grams per mL formula:

Moles x Volume (mL —> L) x Molar mass

Molarity of grams per liter formula:

Grams listed/ (Molar Mass of compound x L)

Hypothesis

Proposes a testable explanation for why something happens

Prediction

The expected outcome if your hypothesis is true

Prediction is phrased as

If…. then….

M Formula

g/L divided by molar mass

W/V Formula:

20g/100mL

A lower pH means a ______ concentration of hydrogen ions.

Higher

A higher pH means a _____ concentration of hydrogen ions.

Lower

pH between 1 to 7 is _____?

Acidic

pH = 7 is ______.

Neutral

pH between 7 to 14 is ______?

Basic

A p-value that’s ______ 0.05 proves statistical significance

Less than

A p-value that’s _____ 0.05 proves statistical insignificance.

More than

What are the graduation units on a serological pipette?

0.1 mL

General Diffusion

movement of solute or solvent down a concentration gradient - from high to low concentration

Osmosis

Diffusion of a solvent (water) across a semipermeable membrane

Dialysis

Diffusion of a solute across a semipermeable membrane (big molecules are blocked, small molecules can pass).

Hypertonic

Water comes out of the cell. (dehydration)

Hypotonic

Water comes into the cell. (Inflation)

Isotonic

Water comes in and out of the cell at an equal rate.

% of survival

(Experiment/ Control) x 100