Enzyme Catalysis Lab

what is the name of the enzyme we used, the substrate and products of the reaction?

enzyeme: catalase

substrate: H2O2

products: h2o2+o2

how would you show that the gas bubbled generated in this lab are oxygen?

using a match o2 will cause the match to relight.

explain the differences in the reactions at 0, 27, and 100 degrees Celsius.

0- the reaction is slow

37- it is faster

100- catalase denatures past optimal temperature.

predict how concentrated sulfuric acid (pH 1.5) would affect the function of catalase. Explain your answer.

sulfric acid likely diffuses catalase, changing its shape and reducing/stopping its function.

design a controlled experiment to test the effect of pH on an enzyeme catalyzed reaction.

test catalase at different pH levels while keeping enzyeme, H2O2, temp, and volume constant. Measure the rr for each pH.

effect of catalase concentration on H2o2 substrate

as enzyme concentration increased, the reaction rate increased. More catalase molecules provided more active sites for h2o2 to bind/break down.

Effect of substrate concentration on reaction times

higher substrate concentration should increase reaction rate.

Temperatures effect

as temperature increases, reaction rate increases then decreases at very hot temperatures. When optimal temperature is reached the enzyme denatures

how to find rate

find the slope, y=mx+b- how fast a reactant is turned into a product

pH effect

also deals with optimal pH (different based on levels) and denatures when reaching a certain pH.